摘要
探讨叶酸代谢通路关键酶基因多态位点与肝细胞性肝癌(Hepatocellular Carcinoma,HCC)发生的相关性.采集205例HCC患者和200例对照人群的血液样本,提取DNA后用SNaPshot技术对叶酸代谢基因多态MTHFRc.677C〉T,c.1298A〉G;MTR-186T〉G,c2756A〉G,+905G〉A;MTRRc56+781A〉G,c66A〉G,CBS-551C〉G进行基因分型及遗传易感性研究.关联分析结果表明:在隐性遗传模式下,MTR启动子区-186G等位基因与肝癌患病风险增加相关(P=0.028),携带G/G基因型的个体患肝癌的风险是G/T和T/T等位基因型个体的1.71倍(OR=1.71,95%CI:1.06~2.78).MTR-186T〉G是肝癌发生的遗传性风险因子.
To investigate the association between SNPs in key genes of folate metabolism pathway and hepatocellular careinoma(HCC). The ease-control study of 8 SNPs were carried out in 205 HCC patients and 200 control participants through SNaPshot. These SNPs included, MTHFR c. 677C〉T, c. 1298A〉G; MTR --186T〉G, c2756A〉G, +905G〉A; MTRR c56+781 A〉G, c66A〉G, CBS--551C〉G. Association study on the 8 SNPs and HCC demonstrated that G allele in rs28372871 was significantly associated with HCC in recessive model(P=0. 028). Individuals with G/G genotype had 1.71-fold risk to be affected by HCC than those bearing C/T and T/T genotype(OR=1. 71,95% CI: 1.06--2.78). G allele in MTR--186T〉G is a genetic risk factor in human HCC.
出处
《复旦学报(自然科学版)》
CAS
CSCD
北大核心
2014年第6期716-723,共8页
Journal of Fudan University:Natural Science
基金
江苏省“十二五医学重点人才”资助项目(RC2011036)
江苏省自然科学基金资助项目(BK2012600)
关键词
肝癌
亚甲基四氢叶酸还原酶
甲硫氨酸合成酶
甲硫氨酸合成酶还原酶
胱硫醚Β合酶
叶酸
hepatocellular carcinoma
methylenetetrahydrofolate reductase
methionine synthase
methioninesynthase reductase
cystathionine β-synthase
folic acid