复合PRP和PPP冻干粉的磷酸钙骨水泥的细胞响应

Cell Response to Calcium Phosphate Cement Incorporating Lyophilized Powders of PRP and PPP

用冷冻干燥制得的富血小板血浆(PRP)和贫血小板血浆(PPP)干粉对磷酸钙骨水泥(CPC)进行改性,采用X射线衍射、扫描电镜和细胞实验等手段研究了PRP和PPP对CPC水化产物物相、断面显微形貌和细胞响应特性的影响.结果表明:复合PRP或PPP并不影响CPC的水化产物,最终水化产物均是弱结晶的羟基磷灰石;复合一定量的PRP或PPP对CPC表面人骨髓间充质干细胞的粘附、增殖和分化具有明显的促进作用,在细胞培养的后期,复合较少的PRP对细胞增殖和分化具有更明显的促进作用.这表明PRP和PPP均有望用于提高CPC的细胞响应能力和骨诱导性.

Calcium phosphate cement （CPC） was modified by incorporating the lyophilized powders of platelet-rich plasma （PRP） and platelet-poor plasma （PPP）, and the effects of PRP or PPP on the hydration product phase, section morphology and cell response of CPC were characterized by means of XRD, SEM and cell experiments. The results show that （ 1 ） incorporating PRP or PPP does not affect the hydration products of CPC, and the final hydra- tion product is nothing hut poorly crystalline hydroxyapatite ; （2） incorporating an appropriate amount of lyophilized powders of PRP or PPP into CPC greatly promotes the adhesion, proliferation and differentiation of human mesen- chymal stem cells （hMSCs） on CPC; and （3） in the late stage of cell culture, incorporating a smaller amount of PRP helps to improve cell proliferation and differentiation. Therefore, it is expected that PRP and PPP can be used to improve the cell response and osteoinductivity of CPC.