金黄色葡萄球菌临床分离株ermA，ermB，ermC和msrA基因检测分析

Frequency of the Occurrence of Genes ErmA,ErmB,ErmC and MsrA Among Staphylococcus Aureus Strains

目的：分析金黄色葡萄球菌对红霉素、克林霉素耐药相关基因与耐药表型的关系，探讨其耐药机制。方法对武汉大学人民医院检验科微生物室分离出的76株金黄色葡萄球菌进行革兰阳性球菌药敏组合试验，同时采用 PCR方法检测 ermA，ermB，ermC和msrA等耐药相关基因。结果76株金黄色葡萄球菌对红霉素和克林霉素耐药相关基因 ermA， ermB和 ermC阳性的有29株，msrA 阳性的有2株。ermA，ermB，ermC 和 msrA 检测与红霉素耐药表型的符合率为82.9％；与克林霉素耐药表型的符合率为89.5％。结论该院金黄色葡萄球菌对红霉素、克林霉素耐药与其耐药相关基因 ermA，ermB，ermC和msrA的编码相关。

Objective To understand the resistant characteristics of staphylococcusaureus and the relationship between eryth-romycin,clindamycin resistance phenotypes and its resistance mechanism.Methods 7 6 Saphylococcus aureus isolated from clinical specimens was identified and antimicrobial susceptibility tested by BD all-automatic microbiology analysis system.U-sing the D-test method to analyzed Erythromycin induced by clindamycin resistance.Genes （ermA,ermB,ermC and msrA） were detected by PCR methods.Results In 76 staphylococcus aureus straims gene ermA,ermB and ermC was found in 29 strains.The msrA gene was present in the 2 strains.The coincidence rate of ermA,ermB,ermC and msrA gene detection with erythromycin was 82.9%.The coincidence rate of ermA,ermB,ermC and msrA gene detection with chindamycin was 89.5%.Conclusion Clindamycin and erythromycin resistance of Staphylococcusaureus is related to the encoding of gene er-mA,ermB,ermC and msrA.