鼻咽癌血清miRNA表达谱分析

Analysis of the serum miRNA expression in nasopharyngeal carcinoma

目的:探索血清中鼻咽癌特异性分子标志物,为其早期诊断提供依据。方法:采用miRNA表达谱芯片分析鼻咽癌患者和正常人的血清miRNA,应用GenePix pro V6.0软件(Axon)进行数据处理与分析,寻找差异表达的miRNA。结果:鼻咽癌患者与正常人比较,血清中2倍升高的miRNA有81个,其中5倍升高的有22个。2倍降低的miRNA有88个,其中5倍降低的有16个。鼻咽癌患者血清中有4个EB病毒相关miRNA(ebv-miRNA)的表达明显升高。结论:鼻咽癌患者血清中存在与鼻咽癌密切相关的miRNA。

Objective： To explore the serum specific molecular markers of the nasopharyngeal carcino- ma and provide the basis for its early diagnosis. Methods： The serum miRNA expressions of naso- pharyngeal carcinoma patients and normal people were analyzed with miRNA chip spectrum, and the data were processed and analyzed with GenePix Pro V6.0 software （Axon）, to find out the dif- ferentially expressed miRNA. Results： Compared with the normal serum, 81 miRNAs increased 2 time high in the serum of pharyngeal carcinoma patients, of which, 22 miRNAs increased 5 times~ while 88 miRNAs decreased 2 times low, of which 16 miRNAs decreased 5 times. And there were 4 EB virus related miRNA （ebv-miRNA） increased significantly in the nasopharyngeal carcinoma pa- tients. Conclusion： Serum miRNA in nasopharyngeal carcinoma patients are closely related to naso-pharyngeal carcinoma.