罗伯逊易位型21三体永生淋巴细胞株的建立

Establishment of immortalized B-lymphoblastoid cell lines of Robertsonian translocation type trisomy 21

目的建立并鉴定13/21易位与21/21罗伯逊易位21三体永生淋巴细胞株,为21三体罗伯逊易位的遗传学研究提供实验材料。方法筛查获得易位型21三体患者并收集外周血,培养B95-8细胞制备EB病毒感染液,采用EB病毒转化法获得永生淋巴细胞株,对第10、15、20代细胞进行G显带染色体分析。结果筛检出1例罕见的13/21易位与4例21/21罗伯逊易位型21三体患儿并成功建立永生淋巴细胞株,传代至第10、15、20代细胞的核型无显著差异。结论 EB病毒转化法可用于易位型21三体永生淋巴细胞株的建立,早期转化的细胞可为该病的研究提供实验基础。

Objective. To establish immortalized B-lymphoblastoid cell lines of Robertsonian translocation type trisomy 21 by Epstein-Barr （EB） virus and conduct karyotype analysis of the cells. Methods： Genetic screening for the Robertsonian translocation type trisomy 21 children; then collection patient＇ s peripheral blood. Cultivation of B95-8 cells was prepared for EB virus transformation of peripheral blood B lymphocytes. Karyotype analysis was performed for the cultured cells of passages 10, 15, and 20 to evaluate their genetic stability. Results： Screening out a rare case of rob （ 13; 21 ） and 3 cases of rob （21; 21 ） . Altogether 5 immortalized lymphoblastoid cell lines with stable chromosome were obtained successfully from the patients. No chromosomal abnormalities were found in the cultured cells of passages 10, 15, and 20. Conclusion. EB virus can be used in the establishment of immortalized B-lymphoblastoid cell lines of Robertsonian translocation type trisomy 21, this chromosomal disease is studied preferably with. cells of early passages.