82株不同来源金黄色葡萄球菌肠毒素的分型检测分析

Analysis of enterotoxin typing detection of 82 strains of Staphylococcus aureus isolated from different sources

目的通过对金华市区日常食品标本、医院病人标本中分离出的金黄色葡萄球菌进行肠毒素分型检测,比较不同来源分离菌株的肠毒素分布差异。方法实验所用的金黄色葡萄球菌为2012年-2013年金华市区日常食品、医院病人标本中分离的菌株。依据GB 4789-2010采用ELISA方法测定金黄色葡萄球菌肠毒素(SEA-SEE)。结果 82株金黄色葡萄球菌中检出62株肠毒素阳性,产肠毒素阳性率为75.6%(62/82),产1种肠毒素和同时产2种及以上肠毒素的菌株分别为24株和30株。日常食品中检出金黄色葡萄球菌40株,29株产肠毒素,阳性率为72.5%;医院病人标本中分离出42株金黄色葡萄球菌,33株产肠毒素,阳性率为78.6%。结论金黄色葡萄球菌产肠毒素能力较强,医院病人分离株和日常食品分离株在肠毒素分布上差异无统计学意义。

Objective To compare the distributional difference of the enterotoxin isolated from Staphylococcus aureus strains from different sources through enterotoxin typing detection of Staphylococcus aureus strains isolated from food and patient samples in Jinhua urban area. Methods Bacteria strains of Staphylococcus aureus were isolated from food and patient samples in Jinhua urban area during 2012- 2013. The type of Enterotoxin was determined according to GB 4789. 10- 2010,using A- E staphylococcal enterotoxin producing assay by enzyme- linked immuno sorbent assay（ ELISA）（ SEA- SEE）. Results Among 82 Staphylococcus aureus isolates,there were 62 strains were found to produce enterotoxin,with an overall positive rate of 75. 6%,including 24 strains in which only one type of enterotoxin was identified,while 30 strains were found to be able to produce 2 different types of enterotoxins. There were 29 strains from 40 Staphylococcus aureus isolated from food samples,with the positive rate of 72. 5%; While 33 strains out of 42 Staphylococcus aureus were isolated from patient samples,the positive rate was78. 6%. Conclusion Staphylococcus aureus had strong ability to produce enterotoxin. The difference of enterotoxin distribution between isolates from patient samples and those from food samples had statistical significance.