应用基因芯片技术研究抗肿瘤传统和天然药物相关文献分析

Literature Analysis on Application of Gene Chip Technology in Antitumor Researches of Natural and Traditional Medicine

目的:为基因芯片技术在传统和天然药物防治肿瘤研究的应用提供参考。方法:查阅2000年1月-2014年1月所有应用基因芯片技术进行抗肿瘤传统和天然药物研究的国内外文献,建立了相应的数据库,从文章发表情况、药物干预种类、研究领域、样品来源、芯片平台、芯片技术重复、差异基因评判标准和验证性等方面总结基于基因芯片技术传统和天然药物防治肿瘤的相关经验和成果,深入分析该技术应用的不足之处。结果:基因筛选实验多局限在细胞水平,应该更加关注动物水平和临床水平的实验;在芯片平台的选择上,学者多关注m RNA的表达水平的差异,而对于基因调控水平和表观遗传研究的micro RNA芯片和DNA甲基化芯片关注度较少;基因芯片实验缺少重复性,直接影响差异基因的评判结果,降低了差异基因筛选的可信度;基因筛选结果的验证,不仅包括m RNA水平的验证,还应该增加蛋白水平的验证。结论:基因芯片是目前最为成熟的基因表达水平检测技术之一,在传统和天然药物抗肿瘤的研究领域应用广泛,研究者在实验设计中如果涉及到了基因芯片技术,应该尽量避免上述的不足之处。

This study was aimed to provide references for techniques in future application of traditional and natural drugs for tumor prevention and treatment. Domestic and foreign literatures on applications of gene chip technology in antitumor studies with natural and traditional medicine from 2000 to 2014 were reviewed. Corresponding database was established from aspects of published articles, drug intervention types, study fields, sample sources, chip technology platforms, repeatability of gene chip experiments, criteria of differential genes, and validation of gene chip experiments. Application experiences of gene chip in antitumor natural and traditional drugs were summarized. Shortcomings of gene chip technology application were analyzed deeply. The results showed that experimental gene screening was limited at the cellular level. More attentions should be paid to experiments at the animal and clinical levels. Scholars had paid more attention to expression level of mRNA and less attention to gene regulation level and epigenetic research of microRNA chip and DNA methylation chip. Gene chip experiments were lack of repeatability, which directly affected the evaluation results of difference gene and reduced reliability of gene screening. Screening results of genes should be verified not only at the mRNA level, but also increased at the protein level. It was concluded that gene chip was one of the most mature technologies to detect the level of gene expression, which was widely used in the research field of traditional and natural antitumor drug studies. Researchers should try to avoid deficiencies mentioned above in experiments related to gene chip technology.