有机阴离子转运多肽3参与全氟辛烷磺酸诱导支持细胞损伤

Organic anion transporting polypeptides 3 is involved in perfluorooctane sulfonate-induced Sertoli cell injury

目的 ：探讨Oatp3在PFOS诱导的支持细胞毒性中的作用。方法 ：体外分离纯化原代大鼠支持细胞,通过细胞毒性实验,观察PFOS对原代支持细胞生长的影响;利用Oatp3 si RNA构建Oatp3基因敲减模型,观察Oatp3在PFOS诱导的支持细胞毒性中的作用,并采用Western blot法检测PFOS对Oatp3蛋白表达的影响。结果：0-30μmol/L剂量的PFOS对大鼠支持细胞生长无明显影响（P〉0.05）;当PFOS剂量增至40μmol/L时,对支持细胞毒性作用明显（P〈0.01）,PFOS毒作用的IC50值约为45.6μmol/L。40μmol/L的PFOS可明显诱导Oatp3蛋白的表达（P〈0.01）,联合Oatp3 si RNA处理后,其诱导作用明显抑制（P〈0.01）。与转染试剂对照组（Mock）相比,加入40μmol/L的PFOS后,支持细胞存活率明显降低（P〈0.01）,联合Oatp3 si RNA处理后,PFOS诱导的支持细胞毒性明显被抑制（P〈0.05或P〈0.01）。结论：Oatp3很可能参与PFOS诱导支持细胞损伤。

Objective：To explore the role of Oatp3 on the PFOS-induced Sertoli cell injury. Methods：The primary Sertoli cell was isolated from 20 day old male SD rat. Cytotoxicity assay,RNAi experiments and immunoblotting analysis were conduced to explore the role of Oatp3 on the PFOS-induced Sertoli cell injury. Results：There is no significant toxic effects observed after PFOS treatment with the dose range from 0-30 μM for 24 hrs（P〉 0.05）. In addition,compared with the control group,40 μmol / L of PFOS significantly decreased the survival rate of Sertoli cells（P 〈0.01）.The IC50 of PFOS on Sertoli cell is 45.6 μmol / L. Further more,40 μmol / L of PFOS significantly increased the expressions of Oatp3 on cells,which was inhibited by Oatp3 si RNA（P 〈0.01）. Compared with Mock group,40 μmol / L of PFOS significantly decreased the survival rate of Sertoli cells（P 〈0.01）,which was inhibited by Oatp3 si RNA（P 0.01）. Conclusion：Oatp3 is involved in PFOS-induced Sertoli cell injury.