以芳姜黄酮为指标的姜黄消痤搽剂质量标准研究

Method of Quality Standard Identification for “Jianghuang Xiaocuo”by Using the Ar-Turmerone as Index

本文用TLC法对姜黄消痤搽剂中芳姜黄酮进行定性鉴别,用HPLC法对芳姜黄酮进行含量测定,使用waters C18高效液相色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(80:20),流速1.0 m L/min,柱温25℃,检测波长242 nm。结果显示TLC色谱中芳姜黄酮斑点清晰,分离效果良好;HPLC法中芳姜黄酮在0.101-3.232μg线性关系良好(R2=0.9995),平均回收率是100.6%,RSD=2.74%(n=6)。表明该方法稳定、可靠,可用于姜黄消痤搽剂的质量控制。

To determine the original quality of Jianghuang Xiaocuo liniment,Ar-Turmerone was identified by TLC and quantified by HPLC,the chromatography was performed on the waters C18 cloumn（4. 6 mm × 250 mm,5 μm） in gradient. The mixture of methyl and water was used as the mobile phase at a flow rate of 1. 0m L / min,the detection wavelength was 242 nm and the column temperature was set at 25℃. The results showed that,TLC spots were clear and well-separated without negative interference. For quantification,the calibration curve of Ar-Turmerone was linear within the range of 0. 101-3. 232 μg（R^2 = 0. 999 5）,the average recovery was 100. 6%,and RSD was 2. 74%（n = 6）,indicating that the established method was simple,accurate,and reliable,thus might be used for quality controlling of Jianghuang Xiaocuo liniment.