口腔黏膜病外周血淋巴细胞亚群的观察及分析

Observation and analysis on lymphocyte subsets of peripheral blood in oral mucosa diseases

目的观察2种常见的口腔黏膜病——复发性阿佛他溃疡(RAU)和口腔扁平苔藓(OLP)患者外周血淋巴细胞亚群分类的变化,探讨其免疫学发病机制。方法用流式细胞术分析105例RAU患者、70例OLP患者和65名健康对照者的外周血淋巴细胞亚群(总T淋巴细胞、CD4+T淋巴细胞、CD8+T淋巴细胞、CD4+/CD8+、NK细胞、B细胞),比较各指标的组间差异。结果与健康对照组相比,RAU组总T细胞和CD8+T细胞显著增加(P<0.05、P<0.01),CD4+T细胞、CD4+/CD8+比值和B细胞均显著减少(P<0.01、P<0.001、P<0.05);而OLP组与健康对照组相比,CD8+T细胞显著降低(P<0.05);RAU组的总T细胞和CD8+T细胞显著高于OLP组(P<0.01、P<0.001)),而CD4+T细胞、CD4+/CD8+比值和NK细胞均显著低于OLP组(P<0.01、P<0.001、P<0.05)。结论 RAU和OLP患者多出现外周血淋巴细胞亚群百分率异常,细胞免疫功能紊乱,但二者的免疫状态存在差异,因此针对性的免疫干预将成为一项极具潜力的治疗措施。

Objective To observe the changes of lymphocyte subsets of peripheral blood in 2 common oral mucosa diseases,recurrent aphthous ulcer(RAU) and oral lichen planus(OLP),and to investigate their immunological pathogenesis. Methods By flow cytometry,the lymphocyte subsets(total T cells,CD4+T cells,CD8+T cells,CD4+/ CD8,NK cells and B cells) in the peripheral blood of patients with RAU(105 cases),OLP(70 cases) and healthy controls(65 cases). The difference was analyzed comparatively between the groups. Results Compared with healthy controls,total T cells and CD8+T cells in RAU group increased(P < 0. 05,P < 0. 01),but CD4+T cells,CD4+/ CD8+and B cells decreased in RAU group(P < 0. 01,P < 0. 001,P < 0. 05). In addition,CD8+T cells in OLP group were lower than those in control group(P < 0. 05). The total T cells and CD8+T cells in RAU group were higher than those in OLP group(P < 0. 01,P < 0. 001),while CD4+T cells,CD4+/ CD8+and NK cells in RAU group were significantly lower(P < 0. 01,P < 0. 001,P < 0. 05). Conclusions Owing to the imbalanced lymphocyte subset percentages,the cellular immune function of patients with RAU or OLP is often found to be abnormal,but their immune states are not completely same. So, the application of selective immunologic intervention may be the promising therapeutic measure to those oral mucosa diseases.