兰州百合组织培养及快速繁殖技术研究

Studies on Tissue Culture and Rapid Propagation of Lifium davidii var.Unicolor(Hoog)Cotton

【目的】建立适于兰州百合(Lilium davidii var.Unicolor(Hoog)Cotton)的快速无性繁殖技术,为食用兰州百合试管苗规模化生产提供技术参考。【方法】以球根鳞片为外植体,采用不同消毒方法、取材部位、接种方法和植物生长调节剂及其浓度配比,筛选适合的诱导、增殖和生根培养基。【结果】球根鳞片先用75%酒精处理后,用10%的次氯酸钠与0.13%的升汞等体积混合液消毒,再用0.13%升汞消毒为最佳消毒方式;接种选择与鳞茎盘相连的下部鳞片为初代培养的最佳外植体;外植体在MS+6-BA 1.5mg/L+GA30.8mg/L+2,4-D 1.0mg/L的培养基中,能诱导出较多长势健壮的不定芽;在MS+6-BA 1.0mg/L+NAA 0.1mg/L的培养基中,增殖倍数达1.25;而生根培养以MS+NAA 0.6mg/L+IBA 0.1mg/L为最佳,生根苗生长良好,生根率达100%。【结论】获得适合兰州百合快速繁殖的培养基配方,建立了兰州百合的快速繁殖技术。

【Objective】The technology of rapid clonal propagation for Lilium davidii var.Unicolor(Hoog)Cotton was established.It can be used for Lilium davidii var.Unicolor(Hoog)Cotton tube seedlings factory production.【Methods】Using the bulb scales as the explants,the effects of different sterilization method,test types,inoculation methods,and plant growth regulators with different concentration on inducement and proliferation were compared.【Results】The results showed that the optimal disinfection method was 75% alcohol treatments followed with the mixture of 10% sodium hypochlorite and 0.1% corrosive sublimate(1∶1)and 0.1% corrosive sublimate.The optimal explants for primary cultured was the lower scales that connect to vittata.The best induction medium was MS+6-BA 1.5mg/L+GA30.8mg/L+2,4-D 1.0mg/L.It could induce more and stronger shoots.The optimal scales medium for subculture was MS with 6-BA 1.0mg/L+NAA 0.1mg/L.The optimal medium for rooting was MS+NAA 0.6mg/L+IBA 0.1mg/L with 100%the rooting rate.【Conclusion】The optimal rapid propagation medium for Lilium davidii var.Unicolor(Hoog)Cotton was identified.The high tissue culture and rapid propagation system of Lilium davidii var.Unicolor(Hoog)Cotton was established.