氦-氖激光穴位照射对脊髓后角SP及ENK的影响——免疫组织化学研究

Influence of laser irradiation at acupoint on substanceP and enkephalin in spinal cord dorsal horn-animmunohistochemistry study

激光照射“穴位”(简称光针)具有镇痛作用,已在临床实践和实验研究中获得广泛证明。光针可以抑制脊髓后角痛敏神经元放电,光针镇痛与其能促进体内吗啡样物质释放有关。 脊髓后角是痛觉调制的初级中枢,有关痛觉传递假说都涉及到脊髓后角内的P物质(SP)和脑啡肽(ENK)。SP与伤害性初级传递有关;ENK是体内吗啡样物质,与痛觉的调制有关。SP与ENK在脊髓后角的形态分布基本相似,提示两者之间在痛觉的传递上存在密切的功能联系。我们观察了光针后大鼠的痛阈变化,并采用免疫组化方法观察了光针后脊髓后角SP和ENK的变化规律,为光针镇痛机理提供了某些依据。

The changes of pain threshold and two neuropetides, substance P (SP) and methionine-enkephalin (ENK), were observed after He-Ne laser irradiating at acupoint(LIA) of rats. The pain threshold was very significantly higher after LIA than before (P<0.001). The SP-LI was obviously enhanced (P<0.001), whereas the ENK-LI markedly diminished (P<0.001) in the rats of laser group as compared with those of control group.