不同来源黄芪药材蛋白质电泳鉴别研究

Identification Study of Astragali Radix of Different Origins by Polyacrylamide Gel Electrophoresis

目的 :探讨利用蛋白质聚丙烯酰胺凝胶电泳(PAGE)技术鉴别黄芪药材的可行性。方法 :超声提取黄芪药材的水溶性蛋白质,对其进行聚丙烯酰胺凝胶电泳,并采用凝胶成像系统分析电泳图谱。结果:黄芪药材的蛋白电泳图谱信息量丰富,不同来源黄芪药材的电泳图谱相似,存在11条明显的共有特征带,同时在条带数目、迁移率和蛋白质相对含量方面存在一定的差异性。结论:PAGE电泳具有分辨率高、谱带稳定、重现性好等优点,可用于黄芪药材的鉴别。

Objectives ：To explore the feasibility of using poIyacrylamide gel electrophoresis（PAGE） to identity radix astragali. Method：Water-soluble proteins in 12 batches of radix astragali were extracted by ultra-sonic and processed with PAGE,and the electrophoretogram was analyzed by using gel imaging system. Results： Electrophoretogram of radix astragali showed rich amount of information. There were obvious similarities in 11 common characteristic bands,while there were differences observed in the number of bands,relative mo- bility（Rf） and relative content of protein. Conclusion：PAGE has many advantages,such as high resolution, stable band and good reproducibility,and therefore it can be used in differentiating radix astragali.