Apc^(Min/+)小鼠红细胞形态及参数分析

An Analysis of Morphology and Parameter of Red Blood Cell in Apc^(Min/+) Mice

[目的]探讨结直肠癌小鼠模型Apc Min/+贫血的类型及病因。[方法]对33只Apc Min/+小鼠和23只同窝对照Apc+/+小鼠进行外周血血细胞计数,比较两组之间红细胞计数(RBC)、血红蛋白含量(HGB)、红细胞压积(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白量(MCH)和平均红细胞血红蛋白浓度(MCHC)间的差异,以及ApcMin/+小鼠上述各项参数与健康小鼠正常值之间的差异。同时,制备外周血血涂片,观察ApcMin/+小鼠红细胞形态的变化。[结果]ApcMin/+小鼠与对照组Apc+/+小鼠的红细胞参数RBC、HGB、HCT、MCV、MCH和MCHC之间均有显著性差异(P均<0.05)。其中,ApcMin/+小鼠的RBC、HGB、HCT、MCH和MCHC各项均值都显著低于对照组,而其MCV的均值显著高于对照组。与之相似,ApcMin/+小鼠的RBC、HGB、HCT、MCV、MCH和MCHC与健康小鼠相比也存在显著性差异(P均<0.05)。Apc Min/+小鼠的血涂片显示易出现大红细胞、嗜多色红细胞、嗜碱性点彩红细胞及网织红细胞。[结论]与对照组Apc+/+小鼠相比,ApcMin/+小鼠的RBC、HGB、HCT、MCH和MCHC的均值降低,而MCV的均值升高,同时红细胞数目减少,提示其为大细胞低色素性贫血。

[Purpose] To investigate the type and pathogenesis of anemia in a colorectal cancer mouse model Apc^Min/＋（multiple intestinal neoplasia）. [Methods] The number of peripheral blood cells,red blood cell count（RBC）,hemoglobin（HGB）,hematocrit（HCT）,mean corpuscular volume（MCV）,mean corpuscular hemoglobin（MCH） and mean corpuscular hemoglobin concentration（MCHC） between Apc^Min/＋mice（n=33） with control mice Apc^＋/＋（n=23） and health mice were analyzed and compared. The morphological changes of blood cells were observed on the blood smear. [Results] There were significant differences between Apc^Min/＋ and Apc^＋/＋mice on red blood cell parameters RBC,HGB,HCT,MCV,MCH and MCHC respectively（all P 0.05）. Among them,RBC,HGB,HCT,MCH and MCHC of Apc^＋/＋mice were significantly lower than those of Apc^＋/＋ mice;however,the mean value of MCV of Apc^Min/＋ mice was higher than that of the Apc^＋/＋ mice. Likewise,there were significantly differences between Apc^Min/＋ mice and healthy mice with regard to RBC,HGB,HCT,MCV,MCH and MCHC（all P0.05）. In addition,there were large erythrocytes,polychromatic erythrocytes,basophilic stippling erythrocytes and reticulocytes on the blood smears of Apc^Min/＋ mice. [Conclusion] Apc^Min/＋ mice exhibited decreases values of RBC,HGB,HCT,MCH,MCHC and increases the number of MCV,indicating Apc^Min/＋ mice have macrocytic hypochromic anemia.