胶孢炭疽菌RGS蛋白生物信息学分析

Bioinformatics Analysis on Regulators of G-protein Signaling in Colletotrichum Gloeosporioides

胶孢炭疽菌侵染众多植物引起的炭疽病,给各国农林业生产造成了巨大经济损失.RGS作为植物病原菌G蛋白信号转导过程中的重要调节因子,在众多生理生化过程中发挥着重要作用.本研究基于前期研究所获得的胶孢炭疽菌中所含有的12个RGS,通过保守结构域、理化性质、疏水性、细胞信号肽、跨膜区结构、亚细胞定位以及二级结构等生物信息学分析,明确上述RGS在保守结构域、理化性质、疏水性、信号肽、跨膜区域、二级结构等方面均具有较大的一致性特点;此外,通过对上述RGS进行遗传关系比较分析,发现不同菌株中的RGS彼此之间具有较近的亲缘关系.该研究为深入开展胶孢炭疽菌RGS功能研究打下坚实的理论基础.

Colletotrichum gloeosporioides can infect many plants, which caused huge economic losses in agriculture and forestry production in many countries. As the important regulatory factor of plant pathogens G protein signal transduction RGS plays an important role in many physiological and biochemical processes. Based on the 12 typical RGS sequences have been re- ported in C gloeosporioides bioinformatics analysis have been analyzed including conserved domain, the signal peptide, trans- membrane domain structure and the secondary structure In addition, analysis of genetic relationships through comparative 12 RGSs in C gloeosporioides each RGS in C. gloeosporioides Cg-14 and C. gloeosporioides Nara gc5 has a high sequence homology and close genetic relationship. The study can provide strong theoretical foundation to research the function of RGS in C gloeosporioides.