HPLC法同时测定宫炎平胶囊中没食子酸、槲皮素、山柰素的含量

Determination of Gallic Acid,Quercetin and Kaempferol in Gongyanping Capsules by HPLC

目的：建立宫炎平胶囊中没食子酸、槲皮素和山奈素的含量测定方法。方法：应用高效液相色谱法测定,色谱柱为Agilent-SB C18（250 mm×4.6 mm,5μm）,以甲醇和0.3%磷酸水溶液为流动相进行梯度洗脱,流速为1.0 ml·min-1,柱温30℃。结果：没食子酸、槲皮素和山奈素的线性范围分别为98.200~491.000μg·ml-1（r=0.999 9）、7.520~37.600μg·ml-1（r=0.999 9）、4.940~24.700μg·ml-1（r=0.999 9）;平均加样回收率分别为96.74%（RSD=1.33%）、98.18%（RSD=1.70%）、97.04%（RSD=1.28%）结论：该方法简便易行、准确、重复性好。

Objective： To establish an HPLC method for the determination of gallic acid,quercetin and kaempferol in Gongyanping capsules. Methods： HPLC was applied with the chromatographic conditions as follows： The chromatographic column was Agilent-SB C18（ 250 mm × 4. 6 mm,5 μm） at 30℃; the mobile phrase was methanol-0. 3% phosphoric acid solution with gradient elution; the flow rate was 1. 0 ml · min- 1. Results： The linearity relationship of gallic acid,quercetin and kaempferol was within the range of98. 200-491. 00 μg·ml- 1（ r = 0. 999 9）,7. 520-37. 600 μg·ml- 1（ r = 0. 999 9） and 4. 940-24. 700 μg·ml- 1（ r = 0. 999 9）,respectively; the average recovery was 96. 74%（ RSD = 1. 33%）,98. 18%（ RSD = 1. 70%） and 97. 04%（ RSD = 1. 28%）,respectively. Conclusion： The method is simple,accurate and repeatable.