高效液相色谱法测定复方消毒剂中聚六亚甲基双胍准确性研究

Study on accuracy of polyhexamethylene biguanide in compound disinfectant by high performance liquid chromatography

目的建立复方消毒剂中聚六亚甲基双胍测定方法。方法采用气体熏蒸载体杀菌试验方法,对机器发生二氧化氯气体杀灭枯草芽孢杆菌黑色变种芽孢高效液相色谱法,对复方消毒剂中聚六亚甲基胍含量测定的准确性和稳定性进行评价。结果用高效液相色谱法检测聚六亚甲基胍含量在5~100 mg/L范围内线性良好。相关系数r=0.9997,回收率为97.8%~100.1%,RSD=1.0%~1.3%,检出限为0.15 mg/L,定量限为0.5 mg/L。结论用高效液相色谱法检测复方消毒剂中聚六亚甲基双胍含量,操作简便、快速准确,可满足日常检测。

Objective To develop the method for determination of Polyhexamethylene biguanide in disinfectants. Methods High performance liquid chromatography was used to evaluate the accuracy and stability for determination of Polyhexam- ethylene biguanide in eompound chemical disinfeetant. Results The calibration curve was linear in the range of 5 - 100 mg/L with the correlation coefficient （r） of 0. 9997, and the recoveries were from 97. 8% to 100. 1% , with the rela- tive standard deviations in the range of 1.0% to 1.3% , the limit of detection was 0. 15 mg/L（S/N =3） , and the limit of quantification was 0. 5 mg/L Conclusion High performance liquid chromatography method is simple, reliable, accurate and available for determination of the Polyhexamethylene biguanide in disinfectants.