PCR-焦磷酸测序法快速鉴定食品中肠炎沙门菌

Rapid detection of Salmonella enteritidis in food by PCR-pyrosequencing

通过对肠炎沙门菌特异性基因片段的焦磷酸测序,达到快速鉴定肠炎沙门菌的目的。设计PCR扩增引物和焦磷酸测序引物,对PCR扩增产物进行焦磷酸测序,测得序列进行BLAST比对分析。通过对肠炎沙门菌特异性基因上的30个碱基片段进行测定,可准确鉴定16株肠炎沙门菌。所建立的方法可以从基因序列水平上准确鉴定肠炎沙门菌,具有快速、准确的优势。

The pyrosequencing method for rapid detection was employed for the specific gene fragments of Salmonella Enteritidis.Amplification primers and sequencing primers were designed for the PCR-pyrosequencing.BLAST results showed that 16 strains were detected in 30 bases of specific gene of Salmonella Enteritidis.The PCR-pyrosequencing method could detect Salmonella Enteritidis at gene level,which has advantages of efficiency and accuracy.