己糖激酶-Ⅱ在胃癌组织中的表达及其临床意义

Expression and clinical significance of hexokinase-Ⅱ in gastric cancer

目的探讨己糖激酶-Ⅱ(HK-Ⅱ)在人体胃癌组织中的表达情况及其临床意义,分析HK-Ⅱ与临床病理特征之间的关系。方法选取2013年3—9月在东南大学医学院附属江阴医院确诊的胃癌病患100例,切除标本,运用免疫组化及RTPCR方法检测胃癌组织及正常胃黏膜组织中HK-Ⅱ的表达情况,并分析其与性别、年龄、浸润深度、TNM分期、分化程度、淋巴结转移、脉管侵犯、肿瘤大小等的关系。结果 HK-Ⅱ在胃癌组织中的阳性表达率为68.00%,显著高于正常胃黏膜组织(P<0.05)。HK-Ⅱ表达与性别、年龄、浸润深度无关(P>0.05);与TNM分期、分化程度、淋巴结转移、脉管侵犯、肿瘤大小有关(P<0.05)。胃癌组织HK-ⅡmRNA阳性率显著高于正常胃黏膜组织(P<0.01)。结论 HK-Ⅱ在胃癌组织中高表达,可作为胃癌的恶性预后指标和临床治疗靶点。

Objective To explore the expression and clinical significance of hexokinase-Ⅱ （ HK- Ⅱ ） in human gastric cancer tissues and to analyze its correlation with clinicopathological charac- teristics. Methods A total of 100 patients with gastric cancers from March to September 2013 were selected, and the expressions of HK- Ⅱ in gastric cancer tissues and normal gastric mucosa tissues were detected by immunohistochemical SP method and RT-PCR. The correlations between HK- Ⅱ and gen- der, age, depth of invasion, TNM staging, differentiated degree, lymphatic metastasis, vascular inva- sion and tumor size were analyzed. Results The positive expression rate of HK-Ⅱ in gastric cancer tissues was 68.00%, which was significantly higher than that in normal gastric mucosa tissues. The HK- Ⅱ expression was not associated with gender, age, depth of invasion （ P 〉 0.05 ）, but was corre- lated with TNM staging, differentiated degree, lymphatic metastasis, vascular invasion and tumor size （ P 〈 0.05 ）. The positive rate of HK- Ⅱ mRNA was significantly higher in gastric cancer tissues than that in normal gastric mucosa tissue （P 〈 0.01 ）. Conclusion HK- Ⅱ, which is highly expressed in gastric cancer tissues, can be considered as malignant prognostic indicators and clinical therapeutic targets for gastric cancer.