YKL-40蛋白检测在卵巢癌诊断中的应用

Application of YKL-40 protein detection in the diagnosis of ovarian cancer

目的检测卵巢癌患者血清人软骨糖蛋白-39(YKL-40)及糖类抗原125(CA125)水平,以及卵巢癌组织YKL-40表达,探讨YKL-40蛋白检测在卵巢癌诊断中的应用。方法选择恶性卵巢癌患者17例,交界性卵巢肿瘤患者16例,以及良性卵巢肿瘤患者19例,另选取非卵巢癌对照患者20例,检测患者术前血清CA125水平及YKL-40水平,术后YKL-40水平,免疫组化检测卵巢组织YKL-40蛋白表达情况。结果术前血清YKL-40水平随肿瘤恶性程度增加而显著升高(P<0.05或P<0.01),卵巢癌患者手术前后YKL-40水平具有显著性差异(P<0.01)。随肿瘤恶性程度增加,肿瘤组织YKL-40表达量增加。YKL-40联合CA125检测诊断恶性卵巢癌的灵敏度显著高于单独检测其中之一。结论 YKL-40蛋白表达可作为卵巢癌的诊断标志物,血清YKL-40水平联合CA125水平检测可提高对卵巢癌诊断的灵敏度。

Objective To detect the serum levels of human cartilage glycoprotein-39（ YKL-40） and carbohydrate antigen 125（ CA125） and the expression of YKL-40 in patients with ovarian cancer so as to explore the application of YKL-40 protein detection in diagnosis of ovarian cancer.Methods A total of 17 patients with malignant ovarian cancer,16 pateitns with borderline ovarian cancer,19 patients with benign ovarian cancer and 20 patients with non-ovarian cancer were selected.The levels of serum CA125 and YKL-40 level before operation and YKL-40 level after operation were detected. Immunohistochemical method was used to detect TKL-40 protein expression in tissues of ovarian cancer. Results YKL-40 level increased along with the malignant severity of ovarian cancer before operation（ P〈0. 05 or P〈0. 01）,and there was significant difference of YKL-40 level before and after operation（ P〈0. 01）. The expression of YKL-40 in tumor tissue increased with the degree of malignancy deterioration. The sensitivity of YKL-40 combined with CA125 detection was significantly higher than that of YKL-40 or CA125 detection. Conclusion Expression of YKL-40 protein can be used as a diagnostic marker for ovarian cancer,and combined detection of YKL-40 and CA125 can improve the diagnostic sensitivity for patients with ovarian cancer.