不同禁欲时间采集精液对IVF-ET实验室指标及临床结局的影响

Effects of semen collection at different abstinence times on laboratory indicators and clinical outcome in IVF- ET

目的：探讨不同禁欲时间采集精液对体外受精-胚胎移植（IVF-ET）实验室指标及临床结局的影响。方法：选取2011年6月~2013年7月在该院生殖中心行IVF-ET治疗符合纳入标准的160例患者（160个周期）为研究对象,根据男方禁欲时间不同随机分为：2～3天组、4～5天组、6～7天组及大于7天组4组,每组40例（40个周期）。比较4组患者洗精前后精液质量、MⅡ卵受精率、正常受精率、正常卵裂率、可用胚胎率、优质胚胎率、临床妊娠率、着床率及早期流产率是否有差异。结果：1随禁欲时间延长4组患者的精液量、精子密度上升（P〈0.05）;精子活率、前向运动精子率及正常形态率逐渐下降（P〈0.05）。而精液经优选技术处理后,以上各指标均有明显改善,处理前后比较差异有统计学意义（P〈0.05）。2 4组患者的MⅡ卵受精率、正常受精率、正常卵裂率、可用胚胎率、优质胚胎率、临床妊娠率、着床率及早期流产率方面比较,差异均无统计学意义（P〉0.05）。结论：IVF-ET中精液质量正常的患者不同禁欲时间采集的精液对实验室指标及临床结局无明显影响。

Objective： To explore the effects of semen collection at different abstinence times on laboratory indicators and clinical outcome in in vitro fertilization and embryo transfer （IVF -ET） . Methods： A total of 160 patients （160 cycles） treated with IVF -ET and meeting the inclusion criteria in Center of Reproductive Medicine in the hospital from June 2011 to July 2013 were selected as study ob- jects, then the patients were divided into 2 -3 -day group, 4 -5 -day group, 6 -7 -day group and 〉 7 -day group, 40 patients （40 cy- cles） in each group. Semen quality, fertilization rates of M Ⅱ oocytes, normal fertilization rates, normal cleavage rates, available embryo rates, high quality embryo rates, clinical pregnancy rates, implantation rates and early abortion rates in the four groups were compared. Re- suits ： With extension of abstinence time, semen volumes and concentrations increased in the four groups （P 〈 0. 05） ; sperm motility rates, forward movement sperm numbers and normal morphology rates decreased gradually in the four groups （ P 〈 0. 05 ） . After being optimized by spermatozoa selection technology, the indicators above - mentioned were improved significantly, there were statistically significant differences between before and after treatment （ P 〈 0. 05 ） . There was no statistically significant difference in fertilization rate of M Ⅱ oocytes, normal fertilization rate, normal cleavage rate, available embryo rate, high quality embryo rate, clinical pregnancy rate, implantation rate and early abortion rate among the four groups （ P 〉 0. 05） . Conclusion： Semen collection at different abstinence times has no significant impact on laboratory indicators and clinical outcome of patients with normal semen in IVF- ET.