柱前衍生高效液相色谱-荧光检测/质谱联用测定沙棘种子中游离脂肪酸

Determination of Fatty Acids in Hippophae rhamnoides L.by Pre-Column Derivatization and HPLC-FLD-APCI/MS

建立了一种利用2-（11-H-苯-a-咔唑）乙基对甲苯磺酸酯（BCETS）作为柱前荧光标记试剂的高灵敏度和高选择性的脂肪酸定量分析方法,并考察了该方法的分析性能。结果表明,方法表现出了较好的重现性和加标回收率,检测限在0.42~1.82ng/mL之间。该方法已被成功的用于青藏高原沙棘种子中脂肪酸含量的分析,结果表明沙棘种子中富含脂肪酸,以不饱和脂肪酸为主,约占总脂肪酸的85.01%。不饱和脂肪酸中,以油酸、亚油酸和亚麻酸为主,分别占总脂肪酸的26.01%、30.86%和14.07%。

In the present study,a selective and sensitive method using 2-（11H-benzo[a]carbazol-11-yl）ethyl 4-methylbenzenesulfonate（BCETS）as the labeling reagent based on pre-column derivatization for the qualitative and quantitative analysis of fatty acids（FAs）was developed and validated by linear range,limits of detection and quantification,precision and accuracy.The proposed method exhibited excellent reproducibility and recovery.This method was successfully applied to the analysis of FAs in the seeds of Hippophae rhamnoides L.（SBT）.The results showed that SBT seeds were rich in FAs,especially for unsaturated FAs（UFAs）,the content of which contributed for 85.01% of the total FAs.As for unsaturated acids,oleic acid,linoleic acid,linolenic acid shareded the majority,their content were26.01%,30.86% and 14.07%,respectively.The analysis of FAs in SBT is important to the pharmacological research of SBT and is also imperative for the quality control.