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透明质酸修饰姜黄素壳聚糖纳米粒的制备及初步细胞毒性考察 被引量：10

Preparation and Preliminary Cytotoxicity Investigation of Hyaluronic Acid Modified Curcumin Chitosan Nanoparticles
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摘要 采用离子交联法制备姜黄素壳聚糖纳米粒,然后通过共价连接将透明质酸连接到纳米粒表面。所得透明质酸修饰姜黄素壳聚糖纳米粒呈球形,平均粒径(205.3±11.4)nm,包封率和载药量为42.7%和28.4%,透明质酸的平均结合率为39%。体外细胞毒性试验显示,该制品对高表达CD44受体的A549细胞增殖的抑制率明显高于未修饰纳米粒和游离药物。而对于CD44低表达的Hep G2细胞,修饰和未修饰纳米粒的抑制率相当。 The curcumin chitosan nanoparticles were prepared by ionotropicgelation method. Then hyaluronic acid was connected to the chitosan by covalent coupling. The hyaluronic acid modified curcumin chitosan nanoparticles were spherical, the average diameter, entrapment efficiency and drug loading of the product were （205.3±11.4） nm, 42.7 % and 28.4 %, respectively. The binding rate of hyaluronic acid to nanoparticles was 39 %. The results of MTT test showed that the proliferation inhibition effect of the product on CD44-overexpressing A549 cells was significantly higher than that of the unmodified nanoparticles or free drug. However, the inhibitions of CD44-unexpressing HepG2 cells proliferation by modified and unmodified nanoparticles were similar.
作者 何媛 张雅溶 李秋霞 王驰
机构地区 重庆医科大学药学院
出处 《中国医药工业杂志》 CAS CSCD 北大核心 2015年第2期162-167,共6页 Chinese Journal of Pharmaceuticals
关键词 姜黄素 壳聚糖 透明质酸 靶向性 细胞毒性 curcumin chitosan hyaluronic acid targeted cytotoxicity
分类号 R944.9 [医药卫生—药剂学]
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共引文献93

同被引文献145

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引证文献10

二级引证文献36

中国医药工业杂志
2015年 第2期

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