黄淮麦区部分小麦品种(系)高分子量麦谷蛋白亚基的SDS-PAGE和分子标记分析

HMW-GS Composition Analysis of Wheat Materials from Huang-Huai Region of China with SDS-PAGE and Molecular Markers

利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和分子标记技术,对黄淮麦区47份小麦育种材料高分子量麦谷蛋白亚基(HMW-GS)进行鉴定和分析。SDS-PAGE结果表明,在所检测的小麦品种(系)中,Glu-A1位点编码的HMW-GS有3种类型,分别是Null、1和2*,其中1出现频率较高(80.9%),Null次之(17.0%),2*仅有1份;Glu-B1位点有7+8、7+9、17+18共3种类型,其中7+8和7+9出现频率较高,分别为48.9%和44.7%;Glu-D1位点有2+12、5+10、4+12共3种类型,其中5+10出现频率最高(61.7%)。利用Dx5、Ax2*、By8、By9和By17亚基特异性分子标记检测结果表明,参试材料中含各标记亚基的材料依次为29(61.7%)、1(2.1%)、23(48.9%)、21(44.7%)、3(6.4%)份;在所检测的小麦品种(系)中含最优亚基组合Dx5、By8的材料共13份,频率为27.7%。分子标记检测结果与SDS-PAGE检测结果相吻合,表明亚基特异性分子标记可用来快速检测小麦材料中的HMW-GS基因。

The types of high molecular weight glutenin subunits （HMW-GS） of 47 wheat materials in Huang Huai wheat area were identified and analyzed by sodium dodecyl sulfate polyacrylamide gel e- lectrophoresis （SDS-PAGE） and molecular marker technology. The results of SDS-PAGE showed that three alleles-encoded HMW-GS （Null, 1 and 2＊ ） were identified at Glu-A1 locus with the highest fre- quency （80. 9%）,followed by Null （17.0%）,2＊ was lowest （only 1 cultivar）;3 alleles at GIu-B1 （7＋8,7＋4-9,17＋18）with the higher frequency （48.9%） at subunit 7--8（44.7%） at subunit 7＋ 9 ; 4 alleles atGlu-D1 （2＋12,5@10,4＋12）with the highest frequency （61.7%） at subunit 5--10. Dx5 , Ax2 ＊ ,By8 ,By9 and By17 genes of tested wheat cuhivars were detected by specific molecular mark ers,and the number of wheat varieties （lines） contained corresponding genes were 29 （61. 7%）, 1 （2.1%） ,23（44.7%）,21（44.7%） and 3（6.4%）,respectively. 13 varieties （lines） with optimal sub unit combinations （Dx5 and By8 ） were identified with frequency of 27. 7% in all tested varieties （lines）. The detection results of molecular markers and SDS-PAGE detection showed that the subunit specific molecular markers could be used for rapid detection of HMW-GS gene in wheat materials.