蜘蛛香提取物对人结肠癌SW480细胞增殖抑制及抗转移作用

Study of Extract of Valeriana Jatamansi Jones Inhibiting Human Colon Cancer SW480 Cells Proliferation and Metastasis

目的:观察蜘蛛香提取物对体外培养人结肠癌SW480细胞增殖抑制及抗转移作用。方法:观察蜘蛛香提取物(400μg/m L)及80μg/m L的5-FU(阳性对照组)对SW480细胞的影响;通过MTT法、细胞脱落实验、病理观察、流式细胞仪检测观察蜘蛛香提取物对结肠癌SW480细胞的增殖抑制、促进凋亡等作用;通过划痕和同质性黏附实验观察其抗结肠癌SW480细胞转移作用。结果:MTT法检测显示蜘蛛香提取物及5-FU均能对SW480细胞产生明显的抑制作用;病理观察发现,经蜘蛛香提取物及5-FU作用后的SW480细胞,呈明显的凋亡状态;流式细胞仪检测发现,蜘蛛香提取物能使S期和G2/M期细胞百分比增加;细胞脱落实验进一步表明,与空白对照组相比,各药物组均能增强细胞的脱落率,抑制细胞的增殖,具有极显著性差异(P<0.01);划痕和同质性黏附实验表明,蜘蛛香提取物能显著降低结肠癌SW480细胞的移动能力(P<0.05)。结论:蜘蛛香提取物对人结肠癌SW480细胞有明显的增殖抑制和抗转移作用。

Objective： To investigate the effect of the extract of Valeriana jatamansi Jones inhibiting human colon cancer SW480 cells proliferation and metastasis. Methods： To observe apoptosis induction and proliferation inhibition effects of the extract（ 400 μg / m L） and 5- FU（ 80 μg / m L） on human colon cancer cells SW480 by MTT assay,cell detachment test,light microscope and flow cytometry test. To observe anti- metastasis effect on colon cancer cells SW480 by scratch test. Results： MTT and cell detachment experiments showed that the extract of Valeriana jatamansi Jones and 5- FU can effectively inhibit the proliferation of SW480. With the light microscope and flow cytometry test,we detected the extract of Valeriana jatamansi Jones can induce the cell apoptosis of SW480 and increase in S phase and G2 /M phase cells percentage,at the same time the extract of Valeriana jatamansi Jones could significantly reduce the mobility of SW480 colon cancer cells in the scratch test. Conclusion： The extract of Valeriana jatamansi Jones has obvious effect of proliferation inhibition and anti- metastasis on human SW480 colon cancer cells.