铜负荷大鼠肝、肾、脑组织Cu含量及其对MT、Aβ影响

Contents of Cu in Copper Overload Rats' Liver,Kidney and Brain Tissues and Its Influence on MT and Aβ

目的:观察铜负荷大鼠肝、肾、脑组织中Cu、Zn、Fe、MT含量及脑组织Aβ含量,并探讨MT、Aβ作用及意义。方法:雄性Wistar大鼠50只,随机均分模型组和对照组,模型组给予含硫酸铜1 g/kg粉状饲料和0.185%硫酸铜水,对照组正常喂饲。第12周结束时,处死动物取血及肝、肾、脑组织标本,ELISA法检测血清中MT含量,等离子体原子发射光谱法检测肝、肾、脑各组织Cu、Zn、Fe含量,免疫组化法检测肝、肾、脑组织MT及脑组织Aβ蛋白表达。结果:与对照组相比,模型组血清MT升高(P<0.05),肝、肾、脑组织Cu含量显著升高(P<0.01),Fe含量降低(P<0.05),Zn无明显差异,肝、肾、脑组织MT及脑组织Aβ蛋白表达显著增加(P<0.01)。结论:铜负荷肝、脑、肾组织Cu及MT含量增高、脑组织Aβ表达增加,MT含量增加可能对组织细胞起到保护作用,同时Aβ表达增加可能与神经元损伤过程有关。

Objective： To observe the contents of Cu,Zn,Fe and MT in brain,liver,kidney and Aβ content in brain tissues in copper overload rats and explore the role and significance of MT and Aβ. Methods： A total of 50 male Wistar rats aged3 months were randomly divided into the model group and the control group. The model group was fed with l g / kg of powder feed containing copper sulfate and 0. 185% copper sulfate water and the control group was given normal feeding. At the end of 12 th week,samples of serum and liver,kidney and brain tissues were obtained from these rats. The contents of Cu,Zn,Fe and MT in blood,liver,kidney and brain tissues and the contents of Aβ in brain tissues were detected.Results： For the model group,the contents of Cu and MT in liver,kidney and brain tissues and the contents of Aβ in brain tissues were significantly higher than those of the control group（ P〈0. 01）. The contents of Fe in liver,kidney and brain tissues were lower than that of the control group（ P〈0. 05）. The contents of MT in blood were higher than that of the control group（ P〈0. 05） and there were no obvious changes in Zn contents. Conclusion： The levels of Cu and MT were increased obviously in liver,kidney and brain tissues of copper load rats. The increased expression of MT may protect the cells while the expression of Aβ may be associated with increased neuronal damage process.