北五味子对糖尿病脑病大鼠脑中神经活性物质含量影响的在线微透析-高效液相色谱-串联质谱联用分析

Effect of Schisandra Fruit on Neurochemicals in Hippocampus of Diabetic Encephalopathy Rat Using Online MD-HPLC-MS/MS

采用在线微透析-高效液相色谱-串联质谱联用方法,测定了糖尿病脑病大鼠大脑海马区的8种脑递质的含量,从脑中神经活性物质的角度研究五味子改善学习记忆能力的作用机制.实验结果表明,经五味子治疗后,痴呆大鼠脑透析液中的谷氨酸(Glu)、丝氨酸(Ser)、多巴胺(DA)及5-羟色胺(5-HT)的含量显著降低(P<0.05),牛磺酸(Tau)及乙酰胆碱(Ach)的含量显著升高(P<0.01),天冬氨酸(Asp)和γ-氨基丁酸(GABA)的含量有降低趋势(P>0.05),8种神经活性物质的水平均向正常水平发生了调节.此结果说明五味子可能通过调节糖尿病大鼠大脑中神经活性物质的含量发挥保护中枢神经系统的作用,从而改善糖尿病脑病大鼠的学习记忆能力.Morris水迷宫实验结果表明,五味子水提物可以明显缩短糖尿病脑病大鼠的逃避潜伏期,增加穿越目标区域次数及中心区域(%)(P<0.05).

The results of morris water maze test showed that the extract of schisandra fruit could significantly shorten the escape latency time（ELT） and increase the time of crossing target zone and central zone（ % ） of diabetic encephalopathy rats. In order to investigate the mechanism of schisandra fruit on the improving ability of learning and memory, online MD-HPLC-MS/MS method was applied to explore the effect of schisandra fruit on eight neurochemicals changes in hippocampus of diabetic encephalopathy rats. The results showed that thecontents of Glu, Ser, DA and 5-HT decreased significantly （P〈0. 05 ）, while the contents of Tau and Ach in- creased significantly（P〈0. 01 ） after treated by schisandra fruit. The contents of Asp and GABA tended to de- crease but the difference was not significant（P〉0. 05 ）. The levels of eight neurochemicals regulated to normal level. The results indicated that schisandra may play a protective function of the central nervous system by adjusting the contents of neurochemicals in diabetic rats, which can improve the memory and learn ability of diabetic encephalopathy rat.