聚乙二醇4000对大鼠创伤性休克早期继发肠损伤的治疗作用

Therapeutic effects of Polyethylene glycol 4000 on the early intestinal injury in rats secondary to traumatic shock

目的探讨聚乙二醇4000（PEG4000）对大鼠创伤性休克早期继发肠损伤的治疗作用及可能机制。方法40只SD大鼠随机分为四组：对照组、休克组、常规复苏组和减阻剂组（静脉输入50μgPEG4000），复苏后2h检测血浆D-乳酸（D—LA）、肿瘤坏死因子-α（TNF-α）含量、肠组织细胞间黏附分子-1（ICAM-1）、丙二醛（MDA）、超氧化物歧化酶（SOD）含量及二胺氧化酶（DAO）活性，计算各组小肠黏膜Chiu’s评分及肠组织湿／干重（W／D）比值。结果与常规复苏组比较，减阻剂组血浆D—LA和TNF-α含量、肠组织ICAM-1和MDA含量、Chiu’s评分及肠组织W／D比值均明显降低，而肠组织SOD含量和DAO活性则明显升高（P均〈0．05）。结论PEG4000可减轻创伤性休克大鼠早期肠损伤，其机制可能与抑制肠组织ICAM-1和TNF-α表达，清除氧自由基及抗脂质过氧化有关。

Objective To investigate the therapeutic effects of Polyethylene glycol 4000 （PEG4000） on the early intestinal injury secondary to traumatic shock in rats and explore its possible mechanisms. Methods Forty SD rats were randomly divided into four groups： control group, shock group, conventional intravenous resuscitation （CIR） group and drag reducing polymers group （DRP group, Ⅳ 50 μg PEG4000 ）. The rats in the four groups were sacrificed two hours after resuscitation. The levels of intercellular cell adhesion molecule - 1 （ ICAM - 1 ）, malondialdehyde （ MDA ）, superoxide dismuctase （SOD） and the activity of diamine oxidase （ DAO ） in the intestinal tissue, plasma levels of D - lactate （ D - LA） and tumor necrosis fator - α （ TNF - α） of rats were detected. Grading of the intestinal mucosal damage （GIMD） and intestinal wet/dry （W/D） weight ratios were measured. Results Compared with those in the CIR group, the levels of ICAM - 1, MDA in the intestinal tissue, plasma D - LA, TNF - α, GIMD and intestinal W/D ratios of rats in DRP group were decreased significantly, while the levels of SOD and the activity of DAO in the intestine tissue were increased significantly （ all P 〈 0. 05 ）. Conclusion PEG4000 can significantly mitigate the early intestinal injury secondary to traumatic shock, and its mechanism may be associated with inhibiting the expressions of ICAM - 1 and TNF - α, scavenging free radicals and anti - lipid peroxidation.