石吊兰总多酚体外抗氧化活性研究

Study on antioxidant activity in vitro of total polyphenol from Lveicnotus pauciflorus Maximl

研究石吊兰总多酚的体外抗氧化活性。采用单因素实验研究提取时间、超声波功率、提取温度、乙醇浓度、提取次数和料液比对总多酚提取率的影响。用还原能力、·OH清除率、DPPH·清除率来考察石吊兰总多酚的体外抗氧化活性。结果表明,超声提取石吊兰总多酚的最佳工艺条件为:提取时间32min,超声波功率为100%,提取温度为25℃,乙醇浓度为80%,提取次数为3次,液料比为20∶1,此时石吊兰总多酚得率为14.0mg GAE/g。此外,石吊兰总多酚的还原能力、对·OH以及DPPH·的清除均高于VC。石吊兰总多酚是天然的抗氧化活性剂和自由基清除剂。

To study the antioxidant activity in vitro of total polyphenol from Lveicnotus pauciflorus MaximI.The single factor was taken to study the effects of extraction time, ultrasonic power,extraction temperature,extraction times, and ethanol concentration liquid-material ratio on the extraction rate of total polyphenoI.The antioxidant activity in vitro of total polyphenol from Lveicnotus paucifiorus Maximl was evaluated by reducing power, hydroxyl and DPPH free radical scavenging effect.The results showed that the optimum conditions for extracting the polysaccharides assisted by ultrasonic wave were as follows：extraction time 32min, ultrasonic power 100%, extraction temperature 25℃,extraction times 3, and ethanol concentration liquid-material ratio 20： 1, and the extraction rate of total poiyphenol was up to ]4.0mg GAE/g.In addition, the reducing power of total polyphenol from Lveicnotus pauciflorus Maximl was more than Vc, and the scavenging ability of DPPH. and · OH was also stronger than Vc.Total polyphenol from Lveicnotus paucifiorus Maximl is an effective and natural antioxidant and free radical scavenger.