不同二氢杨梅素含量的显齿蛇葡萄提取物的抗菌活性与清除DPPH自由基能力研究

Study on the antimicrobial activitiy and scavenging DPPH· capability of extracts from Ampelopsis grossedentata with different content of dihydromyricetin

研究不同二氢杨梅素含量的显齿蛇葡萄提取物的抗菌活性与清除DPPH自由基能力。选取17种二氢杨梅素含量不同的显齿蛇葡萄,使用乙醇超声辅助法获得各个样品的提取物;采用药敏滤纸片法和二苯代苦味酰基自由基（DPPH·）体系分别研究提取物以及相同含量的二氢杨梅素纯品的抗菌活性和清除DPPH自由基能力。结果表明：17种提取物的抗菌活性和清除DPPH自由基能力随着提取物中二氢杨梅素含量的增加而增强,提取物的抗菌活性和清除DPPH自由基能力显著强于相应含量二氢杨梅素纯品。在10mg/m L浓度下,部分提取物的抑菌活性强于二氢杨梅素;对DPPH·清除作用：二氢杨梅素大于全部样品;样品10-17大于VC;样品2-17大于BHT。

The antimicrobial activitiy and scavenging DPPH. capability of extracts of seventeen Ampelopsis grossedentata samples with different content of dihydromyricetin （DMY）were studied.The extracts were prepared from leaves of A.grossedentata under ultrasonic wave-assisted ethanol extraction,the inhibition zone method was used to study the antibacterial activity of extracts, and the DPPH- radical scavenging effect of extracts was investigated using UV spectrophotometer method. In the meantime,antimicrobial activitiy and scavenging DPPH. capability of the contented DMY in extracts and the same concentration of standard DMY were determined to make comparisons. Results showed that all of these extracts had antimicrobial activitiy and scavenging DPPH. capability,and the activities were enhanced with the increasing of DMY content in the extracts.The antimicrobial activitiy and scavenging DPPH. capability of extracts were significantly stronger than the same concentration of DMY. Under the concentration of 10mg/mL,some of the extracts expressed greater antibacterial activity than the same concentration of DMY, and for DPPH- scavenging capability, DMY has stronger than all extracts, extracts （ No.10-17） have better than V~ and extracts（No.2-17） have better than BHT.