精浆果糖速率检测法的初步评价

Evaluation of rate method for detection of seminal plasma fructose

目的建立精浆果糖速率检测法并进行性能评价。方法利用速率法检测精浆果糖浓度,在全自动生化分析仪上建立检测参数,评估该方法的试剂空白吸光度、准确性、重复性和线性范围,与临床常用的精浆果糖吲哚显色法进行比较。结果试剂空白吸光度平均为0.032,空白吸光度变化率(△A/min)平均为0.000 2。3份高、中、低果糖浓度的精浆样本重复测定10次的变异系数(CV)值分别为1.40%、1.57%和2.79%。回收率范围为95.77%~100.97%。精浆果糖浓度在5~35 mmol/L时,具有良好的线性关系(r2=0.997 8)。以精浆果糖吲哚显色法作为对照试剂,速率法作为实验试剂,两者对115例精浆样本的检测结果差异无统计学意义[(20.92±8.62)mmol/L vs(21.15±8.37)mmol/L,t=-1.157,P=0.250],且呈显著正相关(r2=0.939 5,P<0.01)。结论本研究建立的精浆果糖速率检测法有较低的试剂空白,重复性和准确性较好,与目前临床使用的吲哚显色法有很好的一致性。本法操作简单、快速,精浆样本无需稀释,适合临床大批量样本的检测筛查,可节省人力和试剂成本。

Objective To establish rate method for determination of seminal plasma fructose and evaluate its performance. Methods The fructose concentration in seminal plasma was determined by rate method and the detecting parameters for the automatic biochemical analyzer were set up. The reagent blank absorbance, accuracy, repeatability and linear range of the automatic method were evaluated and the results of the method were compared with those of indole chromogenie method commonly used in clinical laboratories. Results The average absorbance of the reagent blank was 0.032 and the average change rate of blank absorbance （z^A/min） was 0. 000 2. The coefficients of variation （CV） in 10 repeated determinations for 3 seminal plasma samples with high, middle and low fructose con- centration, were 1.40% , 1.57% and 2.79% , respectively. The recovery rates ranged from 95.77% to 100.97%. A good linear re- lationship （ r2 = 0. 997 8 ） was shown when the concentration of seminal plasma fructose was between 5 and 35 mmoL/L. Compared with the results of indole chromogenic method, the rate method showed no statistical difference （[20.92 ± 8.62] mmog/L vs [21. 15± 8.37] mmol/L, t = - 1. 157, P =0. 250） and the results of 115 seminal plasma samples showed significant positive correlation （r2 = 0. 939 5, P 〈 0.01 ） between the two methods. Conclusion The established rate method in the determination of seminal plasma fructose concentration showed low reagent blank, good repeatability and accuracy, and fine concordance with the colorimetric method used routinely in clinical laboratories. The method should be simple, rapid, and suitable for screening large numbers of samples, and greatly saves the resources of manpower and reagents. The dilution of seminal plasma samples is unnecessary for the determination.