白细胞介素-13基因多态性与中国儿童支气管哮喘易感性关系的Meta分析

Meta - analysis on the association of interleukin - 13 gene polymorphism and the genetic susceptibility of asthma in Chinese children

目的 采用 Meta分析方法对已发表的白细胞介素-13（IL-13）基因多态性与中国儿童支气管哮喘（简称哮喘）易感性关系的文献进行综合分析，评价 IL-13基因多态性与中国儿童哮喘易感性的关系。方法 计算机检索Medline数据库、Embase数据库、Ovid数据库、Cochrane图书馆及中文生物医学期刊数据库等网络资源，并查阅所有纳入文献的参考文献，全面收集建库至2014年4月发表的IL-13基因多态性与中国儿童哮喘相关性的病例对照研究，对采纳的数据进行Meta分析。结果 18篇文献被纳入分析，其中3篇文献涉及C-1112T多态性，6篇文献涉及C ＋1923T多态性，14篇文献涉及G ＋2044A多态性。Meta分析计算合并比值比（OR），IL-13 -1112C/T多态性与中国儿童哮喘易感性无相关性（OR =1.00，95% CI0.82～1.22，P =0.98），TT/CC基因型OR =1.15（95% CI0.57～2.33，P =0.69），TC/CC基因型OR =1.01（95% CI0.82～1.25，P =0.89）。IL-13 ＋1923C/T多态性与中国儿童哮喘易感性呈正相关（OR =1.86，95% CI1.29～2.67，P =0.0009），TT/CC基因型OR =2.12（95% CI1.27～3.56，P =0.004），TC/CC基因型 OR =1.67（95% CI1.18～2.35，P =0.003）。IL-13 ＋2044G/A多态性与中国儿童哮喘易感性无相关性（OR =1.33，95% CI0.94～1.88，P =0.11），AA/GG基因型OR =1.30（95% CI0.76～2.20，P =0.34），AG/GG 基因型 OR =1.24（95% CI0.90～1.70，P =0.19）。结论 IL-13 ＋1923位点基因型TT、TC是中国儿童哮喘发病的危险因素，与中国儿童哮喘易感性密切相关，其中T等位基因能增加哮喘发病的风险。IL-13 -1112位点基因型 TT/TC及 IL-13 ＋2044位点基因型 AA/AG 与中国儿童哮喘发病尚无明确关系。

Objective Published literatures on the relationship between IL -13 gene polymorphism and the susceptibility of children to bronchial asthma in China were comprehensively analyzed with the use of Meta - analysis to evaluate this relationship. Methods The data were collected from the Medline database, Ovid database, the Cochrane library, and Chinese Biomedical database, and the references of eligible studies were manually screened. Published data related to case - control studies reporting the link between IL - 13 polymorphisms and asthma in Chinese children were retrieved through those database. Meta - analysis was conducted to determine whether the IL - 13 gene polymorphisms were associated with asthma. Results Eighteen studies were finally accepted for analysis. There were three studies fo- cusing on C -1112T polymorphism ,and six studies focusing on C ＋ 1923T polymorphism,and fourteen studies focusing on G ＋ 2044A polymorphism. There was no evidence to confirm that the genotypes in position IL -13 - 1112 C/T were associated with asthma in Chinese children [ odds ratio （OR） = 1.00,95 % CI 0. 82 - 1.22, P = 0.98 ]. The OR of asth- ma for TT/CC genotypes was 1.15 （95 % C10. 57 -2.33, P = 0. 69 ） and for CT/CC was 1.01 （95 % CI 0. 82 - 1.25, P = 0.89 ）. There was significant evidence to confirm that the genotypes in position ＋ 1923 C/T were associated with asthma in Chinese children （ OR = 1.86,95 % CI 1.29 - 2.67, P = 0.000 9 ）. The OR of asthma for TY/CC genotypes was 2.12（95% CI 1.27 -3.56 ,P =0. 004） and for TC/CC was 1.67（95% CI 1.18 -2.35 ,P =0. 003）. There was no correlation between IL - 13 ＋2044G/A polymorphism and the susceptibility（ OR = 1.33,95% CI 0. 94 - 1.88,P = 0.11 ）. The OR of asthma for AA/GG genotypes was 1.30（95% CI 0. 76 -2.20,P =0.34） and for AG/GG was 1.24 （ 95 % CI 0. 90 - 1.70, P = 0.19 ）. Conclusions IL - 13 gene ＋ 1923 Tr and TC genotypes should be associated with susceptibility of asthma in Chinese children, and the T allele could increase the risk of asthma. No clear relationship was found between the genotype TT/TC at the IL - 13 - 1112 site and the incidence of asthma of children in China, and so was the genotype AA/AG at the IL- 13 ＋2044 site and the incidence.