贵州半夏块茎腐烂病病原菌的分离与鉴定

Isolation and identification of pathogens from rotted root of Pinellia ternata in Guizhou province

【目的】对贵州省半夏块茎腐烂病病原菌进行分离和鉴定,为开发有效的防治技术体系提供依据。【方法】采用组织分离法进行病原菌分离,根据柯氏法则进行致病性测定,结合形态学、生理生化特性和分子生物学方法进行鉴定。【结果】从贵州3个半夏产地患病样品中分离出12株病原细菌和5株病原真菌。经形态学、生理生化特性和分子生物学方法鉴定,12株病原细菌为胡萝卜软腐果胶杆菌胡萝卜软腐亚种;ZJ、Z3病原真菌为尖孢镰刀菌,D3、D5、H1为茄病镰刀菌。【结论】贵州省半夏块茎腐烂病的病原菌有病原细菌和病原真菌。

[Objective] To identify microbes from rotted root ofPinellia ternata, so as to provide clues for efficient prevention of this plant disease. [Methods] Pathogens were isolated and their pathogenicity was confirmed by Koch＇s role. These pathogens were further studied through etiological, morphological and bacteriological techniques. In addition, 16S rRNA sequence analysis was done for bacterial pathogens, ITS and TEF sequence were used analysis for fungal pathogens. [Results] Twelve strains of bacterial pathogens and five strains of fungal pathogens were found in rotted tuber of P. ternata from three different cultivating places in Guizhou. The twelve strains of bacterial pathogens belonged to Pectobacterium carotovorum subsp, carotovorum. Among the fungal pathogens, ZJ and Z3 belonged to Fusarium oxysporum, and D3, D5 and H1 belonged to F. solani. [Conclusion] The pathogens that cause root-rot of Pinellia ternata in Guizhou include bacterial pathogens and fungal pathogens.