利用高效液相色谱法建立玉屏风总苷的指纹图谱

Establishment of fingerprint of total glucosides of Yupingfeng by HPLC

目的：以黄芪皂苷Ⅱ为参照峰,建立玉屏风总苷HPLC-ELSD的指纹图谱,对玉屏风总苷进行质量控制。方法：采用HPLC-ELSD法,色谱柱为Agilent C18（250 mm×4.6 mm,5μm）;以乙腈和0.1%甲酸为流动相进行梯度洗脱,柱温40℃,流速1.0ml·min^-1,漂移管温度110℃,N2载气流速3.30 L·min^-1。结果：建立了玉屏风总苷的HPLC-ELSD的指纹图谱,确定了17个共有峰,并鉴定了其中4个共有峰,各峰相对保留时间的RSD在0.1%-0.75%之间,相对峰面积的RSD在1.68%-4.82%之间。10批总苷样品的相似度均大于0.94。结论：该法简单、精密度、稳定性、重现性好,适用于玉屏风总苷的指纹图谱检测。

OBJECTIVE To establish fingerprint of total glucosides of Yupingfeng by AstragalosideⅡ as the reference peak,and control the quality of total glucosides of Yupingfeng.METHODS HPLC-ELSD was used on an Aglient column（4.6 mm×250 mm,5μm）,with gradient mobile phase composed of acetonitrile and 0.1%formic acid.The column temperature was set at40 ℃ and flow rate of mobile phase 1.0mL·min^-1.The tube temperature of detector was 110 ℃,and flow rate of N2 was 3.30L·min^-1.RESULTS HPLC-ELSD fingerprint of the total glucosides of Yupingfeng was established,which determined 17 common peaks from10 batches of the total glucosides of Yupingfeng and indentified 4 of them.RSD values of relative retention times for peaks and peak areas were respectively in the range of 0.1%-0.75% and 1.68%-4.82%.The similarity of 10 batches of total glucosides was all greater than 0.94.CONCLUSION The established HPLC-ELSD fingerprint is simple and has desirable precision,stability,reproducibility,and it can be applied to determine the fingerprints of total glucosides of Yupingfeng.