人参中41种有机磷农药多残留分析方法

Analytical Method for Determination of 41 Organophosphorus Pesticide Retention in Ginseng

目的：建立人参中41种有机磷农药多残留的气相色谱分析方法。方法样品以乙腈为提取剂,PSA固相萃取柱（填料为N-丙基乙二胺）净化,丙酮-正己烷洗脱,采用火焰光度检测器（FPD）检测和ZB-1701毛细管柱分离,程序升温,进行气相色谱测定。结果41种有机磷农药在5～1000μg/kg范围内线性关系良好,相关系数在0.991～0.998之间。在50、100、500μg/kg浓度水平的平均回收率在69.8%～99.7%之间,RSD在1.9%～15.3%之间。各种农药的定量限在9～45μg/kg。结论本方法准确度高,灵敏度好,操作简便。

Objective To establish a gas chromatographic method for the determination of 41 organophosphorus pesticide retention in ginseng. Methods Samples were extracted with acetonitril, purified by PSA solid phase extraction column, eluted by acetone-normal hexane, detected by FPD, and separated by ZB-1701 capillary column. Temperature for the samples was increased through program and the samples were determined by gas chromatography. Results 41 organophosphorus pesticides showed good linear relation in the range of 5-1000 μg/kg, and correlation coefficient was 0.991-0.998. The average recoveries at the three fortification levels （50, 100, 500μg/kg） were between 69.8% and 99.7% with the RSD between 1.9% and 15.3%. The limits of quantification （LOQ） of the 41 pesticides were between 9μg/kg and 45μg/kg. Conclusion The method developed in this study is with high degree of accuracy, good sensitivity, easy and simple to handle.