杨树多基因遗传转化体系的建立及优化

Establishment and Optimization of Multi-gene Transformation System of Populus

为建立多基因遗传转化体系,以‘宁杨1号’为转化受体材料,建立杨树多基因遗传转化体系,并对SOS1、SOS2、SOS3进行了共转化。研究结果表明:MS+6-BA 1.0 mg/L+NAA 0.5 mg/L为最适不定芽分化培养基;最佳的农杆菌侵染浓度为OD600=0.4,最适除草剂筛选浓度为0.8 mg/L。建立了最佳的‘宁杨1号’多基因遗传转化体系,获得14株PCR阳性株系。

In order to establish and optimize the multi- gene transformation system of Populus,‘Ningyang No.1＇was used as acceptor material, and multi-gene transformation system was established. SOS1, SOS2 and SOS3 genes related to salt resistance were co-transformed. The results showed that the most suitable medium of adventitious bud was MS＋ 6-BA 1.0 mg/L＋ NAA 0.5 mg/L, the most suitable screening concentration of herbicide for the leaf piece was 0.8 mg/L, and the most suitable agrobacterium suspension concentration was OD600=0.4. The 14 lines of PCR positive were obtained using this system.