抑制杨树烂皮病高效菌株的筛选及分类地位验证

The Screening and Physiology Characteristic of Efficient Antifungal Strain on Cytospora chrysosperma

为了寻找对杨树烂皮病病原菌具有高效抑制作用的木霉菌株,采用对峙培养法和生长速率法对国内外29个木霉菌株进行了筛选,并对筛选出的高效菌株的生理生态特性和分类地位进行了研究。结果表明:菌株T-33发酵液正丁醇提取物对病原菌菌丝生长的抑制率最高,达到94.2%,对孢子萌发抑制率达到100%。菌株T-33生长最适碳源为葡萄糖,最佳用量为3%(g/L);最适氮源为黄豆粉,最佳用量为0.02%(g/L);培养基最适初始p H 6;最适培养温度25℃。通过18S r DNA测序分析,ITS序列长度是583 bp,菌株T-33鉴定为Trichoderma viride。

In order to look for the Trichderma strains which have inhibiting effects on Chtospora chrysosperma,29 domestic and foreign strains were screened by methods of antagonistic culture and growth-inhibiting. The morphological characteristics and physiological characteristics and taxonomic status of efficient antifungal strain were studied. The results showed that the growth-inhibiting rate of the N-butanol extract extracted from the fermented liquid of strain T-33 was 94%, and inhibition rate of spore germination was 100%. The optimal C-source of strain T-33 was glucose and the optimal content was 3%（g/L）. The optimal N-source of strain T-33 was soy flour and the optimal content was 0.02%（g/L）. The optimal initial p H of culture medium was 6.The optimal culture temperature was 25℃. Through 18 S r DNA sequencing and phylogenies analysis of T-33,the length of ITS sequence was 583 bp, strain T-33 was verified as Trichoderma viride.