‘早胜牛’微卫星基因位点遗传多样性分析

The Analysis of Genetic Diversity of Microsatellite Loci in‘Zaosheng Cattle'

为了从分子水平上分析‘早胜牛’的遗传多样性,采用PCR技术和非变性聚丙烯酰胺凝胶电泳技术检测了‘早胜牛’IDVGA46、ILSTS005、IDVGA27、TGLA44、IDVGA2、BM2113和ETH10这7个微卫星位点的多态性分布,并计算‘早胜牛’各个微卫星位点基因座的等位基因数、有效等位基因数、期望杂合度、多态信息含量。结果显示:112头‘早胜牛’样品在7个微卫星位点上共有25个等位基因,平均等位基因数为3.5714,平均有效等位基因数为3.0004,平均期望杂合度为0.6996,平均多态信息含量为0.6526。分析结果表明‘早胜牛’群体的遗传杂合度比较高,遗传多样性丰富,所选的微卫星座位点可用于‘早胜牛’遗传多样性评估。

To analyze the genetic diversity of ＇Zaosheng cattle＇ in the molecular level, polymorphism distributions of microsatellite loci which including IDVGA46, ILSTS005, IDVGA27, TGLA44, IDVGA2, BM2113 and ETH10 were detected in ＇ Zaosheng cattle＇ by Polymerase Chain Reaction and Polyacrylamide Gel Electrophoresis. Number of alleles and effective alleles, expected heterozygosity and polymorphic information content were calculated. The results showed that a total of 25 alleles were identified on the 7 microsatellite loci with polymorphism from 112 samples. The average number of alleles and effective alleles was 3.5714 and 3.0004, respectively. The average expected heterozygosity was 0.6996 and the average polymorphic information content was up to 0.6526. The results indicated that genetic heterozygosity of ＇ Zaosheng cattle＇ was relatively high, and the genetic diversity was rich. The selected microsatellite loci could be used to evaluate genetic diversity of ＇Zaosheng cattle＇.