上海地区水稻已知品种SSR指纹图谱库构建

Establishment of DNA Fingerprint Database of Rice Varieties in Shanghai

用24对引物构建上海地区53份水稻品种的DNA指纹图谱,为当地水稻新品种保护、遗传资源评价及亲缘关系分析提供理论依据和技术支持。结果显示,试验中共检测出109条多态性片段,变幅2~9条,平均每对引物4.5417条多态性片段,有效等位基因数为70.8187,平均每个标记2.9508个;Shannon多样性指数（Ⅰ）平均值为1.1296,变幅0.4949~1.8402;多态性信息含量（PIC）平均值为0.5415,变幅0.2656~0.8001。用24个标记建立53份材料的DNA指纹图谱,这些字符串就构成了这53份材料的＂身份证号码＂,本试验中,各材料的分子身份证号码是唯一的;聚类分析表明,在遗传相似系数0.21处可以将53份材料分为2个类群,分别为粳稻组和籼稻组,但是组内遗传距离较近。由此可见,上海地区水稻品种的遗传多样性相对狭窄。

The DNA fingerprints can provide a theoretical foundation and technical support for the variety＇ s protection, evaluation of genetic diversity and relationships analysis. In this study, the DNA fingerprints of 53 rice varieties in Shanghai were established by 24 pairs of SSR primers. A total of 109 alleles were detected from tested rice varieties, ranged from 2 to 9. The average number of alleles per locus was 4.5471. The effective alleles was 70.8187, 2.9508 for each primer pair on average. Shannon＇ s information index （ I ） ranged from 0.4949 to 1.8402 with average 1.1296. And its average PIC index was 0.5415, with a range of 0.2656-0.8001. 24 pairs of SSR were used to establish the DNA fingerprints, and the strings constituted the ＇ID number＇. In this experiment, the ID number was unique for every cultivar. The cluster analysis showed that the tested cuhivars could be classified into 2 groups at the level of genetic similarities coefficient 0.21, which were Japonica group and Indica group. The results indicated that narrow genetic base existed among the detected cuhivars in Shanghai.