蜡样芽孢杆菌用于苎麻脱胶研究

Research on Bacillus cereus for the Degumming of Ramie

通过定期测定高效菌株L11在对苎麻进行脱胶处理过程中发酵液中有机物的相关含量,探究其变化规律,阐明苎麻生物脱胶机制。结果表明,在脱胶前期,残胶率降低幅度较大,中期趋于平缓,后期下降幅度再次增大;脱胶前期和中期脱胶菌活菌数量不断增加,后期有下降趋势;脱胶前期果胶酶和木聚糖酶酶活增加缓慢,中期迅速增加,后期成下降趋势,纤维素酶酶活很低,变化不大;p H脱胶前期和中期降低,后期增加,呈"V"字型;蛋白质含量先增后降,出现1个峰值;还原糖出现2个峰值,呈"M"型变化。

In this paper, the contents of organics in the zymotic fluid during the ramie degumming process with high efficient bacteria L11 were measured periodically. Their change rules were also analyzed for studying the mechanism of biological degumming of ramie. The results showed that, the residual gum rate decreased greatly in the early period of degumming, flattened out in the middle period and decreased sharply again in the late periods of degumming. The quantity of live degumming bacteria increased continually in the early and middle periods of degumming and tended to decrease in the late period. The enzyme activity of pectinase and xylanase increased slowly in the early period, increased rapidly in the middle period and tended to decrease in the late period of degumming. The enzyme activity of cellulose was very low and changed little in the degumming process. The pH value decreased in the early and middle period and increased in the late periods, like a ＇V＇ shape. The content of protein firstly increased and then decreased, it showed a peak value. The curve of reducing sugar showed two peak values and varied like an ＇M＇ shape.