高浓度LPS损伤巨噬RAW264.7线粒体功能的研究

Study on the high concentration of LPS damaging macrophage RAW264. 7 mitochondrial function

目的研究不同浓度LPS对巨噬细胞线粒体功能的影响。方法体外培养小鼠巨噬细胞RAW264.7,不同浓度LPS(0、1、2、4、8和16μg/m L)诱导24 h后,采用流式细胞术检测线粒体功能,并分析细胞凋亡和细胞内活性氧水平。结果低浓度的LPS促进细胞内活性氧产生,对线粒体功能无影响或具有略增强的效应;高LPS诱导下,活性氧水平较低浓度组下降,而线粒体功能受损伤、坏死和凋亡细胞比例增加。结论高浓度LPS对RAW264.7线粒体功能有损伤作用。

Objective To study effect of different concentration LPS on macrophages mitochondrial .Methods Mice macrophage RAW264.7 was cultured in vitro and induced by different concentration of LPS （0,1,2,4,8 and 16 μg/mL） for 24 hours.Mitochondrial function was detected by flow cytometry and cell apoptosis and active oxygen level in the cell were analyzed .Results Intracellular reactive oxygen could be promoted by low concentration of LPS and there was no or slightly effect on enhancing mitochondrial function;the level of active oxygen concentration was de-creased with high concentration of LPS and mitochondrial function was damaged ,necrosis and apoptosis cell percentage increased.Conclusion RAW264.7 mitochondrial function can be damaged by high concentration of LPS .