熊去氧胆酸对人肝内胆管瘢痕成纤维细胞生长影响的研究

The effects of ursodeoxycholic acid on scar fibroblast growth in human intrahepatic bile ducts

目的 探讨熊去氧胆酸（UDCA）对人肝内胆管瘢痕成纤维细胞生长的影响。方法 原代培养人肝内胆管瘢痕组织成纤维细胞。通过显微镜观察和免疫荧光染色鉴定细胞,采用噻唑蓝（MTT）法和划痕实验检测UDCA对瘢痕成纤维细胞生长的影响。结果 ①成功原代培养人肝内胆管瘢痕成纤维细胞;②MTT显示：CA和DCA浓度在0.5∽10.0μmol/L之间促进细胞活力,而UDCA浓度大于5μmol/L时,则明显抑制细胞的活力（〈0.05）。随着UDCA浓度升高,CA组和DCA组细胞活力逐渐下降;③划痕实验结果显示：随着UDCA浓度升高,CA组和DCA组细胞愈合距离逐渐增加。结论 UDCA可能抑制CA和DCA的促瘢痕成纤维细胞生长的作用。

[ Objective ] To discuss the effects of UDCA on scar fibroblasts growth of human intrahepatic bile ducts. [ Methods ] The primary scar fibroblast cells in human intrahepatic bile ducts were cultured . The cells were identified by cell morphology and immunofluorescence staining. The growth of scar fibroblast was tested by MTT method and Wound-Healing. [ Results ] （1）Scar fibroblasts in human intrahepatic bile duct were successfully cultured. （2） MTT results showed in the concentration of 0.5 to 10 μmol/L, CA and DCA increased the scar fibroblast viability, 〉20μmol/L, UDCA inhibited the growth of scar fibroblasts. With increasing concentration of UDCA, the cell viability of CA group or DCA group was gradually declined. （3）With increasing concentration of UDCA, the dis- tance healing of the CA group and DCA group was gradually increased. [ Conclusions ] UDCA may inhibit CA and DCA from promoting scar fibroblasts growth.