T细胞受体保守域α链基因-575A/G多态性与终末期肾病微炎症状态的相关性研究

Study on the relationship between the TCRCα-575A/G polymorphism and microinflammatory state of end-stage renal disease

目的探讨T细胞受体保守域α链基因(TCRCα)-575A/G多态性与终末期肾病微炎症状态的相关关系。方法利用聚合酶链式反应-限制性片段长度多态性分析法(PCR-RFLP)来鉴定基因型,以检测终末期肾病患者和正常对照组TCRCα基因-575A/G多态性分布规律。并收集临床指标,进行病例对照研究及临床资料分析。结果 1在终末期肾病组和对照组,均检测出A、G两种TCRCα基因-575A/G等位基因,三种基因型AA型、AG型、GG型;2终末期肾病组和正常人对照组相比,基因型和等位基因分布频率均无统计学意义(P>0.05);3临床资料显示:终末期肾病患者的CRP、Lp(a)与AngⅡ的水平均比健康对照组高(P<0.05),差异有统计学意义。终末期肾病组,三种基因型在年龄、性别、Lp(a)、AngⅡ水平方面相比,差异无统计学意义(P>0.05),GG基因型的终末期肾病患者的CRP水平较AG和AA基因型明显升高,差异有统计学意义(P<0.05)。正常对照组中,三种基因型在年龄、性别、CRP、Lp(a)和AngⅡ水平方面相比,差异无统计学意义(P>0.05)。结论终末期肾病普遍存在微炎症状态,TCRCα-575A/G基因多态性可能与CRP的水平相关,与终末期肾病的发病易感性不相关。

[Objective] To investigate the clinical relationship of TCRCα-575MG polymorphism with microin- flammatory state of end-stage renal disease. [ Methods ] The genotypes of TCRCαgene polymorphism （-575A/G） was identified by PCR-RFLP to detect TCRC -575A/G gene polymorphism distribution between ESRD patients and nor- mal control group, and the clinic information was obtained for analysis of case-control study and clinical data. [Result] （1）TCRCαgene （-575A/G） included 3 genotype （AA,AG,GG） and 2 allele gene （A or G） in two groups; （2） There were no statistical significant difference of genotypes distribution and allele frequency between ESRD group and the control （P 〉0.05）; （3）The CRP, lp （a） and Ang ]I level of ESRD group were higher than the control, and the difference was statistically significant （P 〈0.05）. The CRP level in the GG genotype was higher than the AG OR AA genotypes （P 〈0.05）, and no correlation was found in the -575A/G polymorphism of TCRCα with sex, age, lp（a） and Ang Ⅱ level （P 〉0.05） in ESRD group. No correlation was found in the -575A/G polymorphism of TCRCα with CRP, lp（a） and Ang Ⅱ level （P 〉0.05） in the control. [ Conclusion ] Microinflammatory state was common in end stage re- nal disease patients. TCRCα-575A/G polymorphism may be associated with the CRP level. However, this polymor-phism was not associated with susceptibility to ESRD.