羊耳菊药材的HPLC指纹图谱研究

Researching on fingerprint of Inulacappa by HPLC

目的:建立羊耳菊药材的高效液相指纹图谱,并对共有色谱峰进行指认,为其质量控制方法提出科学依据。方法:采用HPLC-DAD检测法,Agilent Eclipse Plus C18柱(2.1 mm×150 mm,1.8μm);流动相0.1%甲酸水-0.1%甲酸乙腈溶液梯度洗脱;柱温45℃;检测波长325 nm;流速0.3 m L·min-1。结果:对10批羊耳菊药材进行测定,标定了17个共有峰,指认了其中5个共有峰,10批羊耳菊药材的相似度均在0.95以上。结论:该方法可靠、简便,可用于羊耳菊药材的质量评价。

Objective： This study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC. Method： The HPLC analysis was performed onan Agilent Eclipse Plus Cls column（2.1 mm × 150 mm, 1.8 μm） with 0. 1%fo- mic acid aqueous solution-0.1% fomic acid acetonitrile solution as mobile phase at a flow rate of 0.3 mL · min -1 ; The detective wavelength is 325 nm; The column temperature is 45℃. Result： The results indicated that 5 of 17 common peaks were identified . The similarity about 10 groups of Inulacappais is over 0.95. Conclusion： This method is able to be a scientific basis of quality assessment according to its convenient and reliable.