固相萃取-高效液相色谱法双检测器同时测定水中莠去津和苯并(a)芘

Simultaneous determination of atrazine and benzo(a) pyrene in water by solid phase extraction-high performance liquid chromatography with double detector

目的建立固相萃取-高效液相色谱法双检测器同时测定水中莠去津和苯并（a）芘分析方法。方法用大体积固相萃取柱富集莠去津和苯并（a）芘,二氯甲烷洗脱,使用Eclipse XDB-C18分析柱（150 mm×4.6 mm,5μm）,采用乙腈-水体系作为流动相,梯度洗脱,二极管阵列检测器（DAD）和荧光检测器（FLD）检测。结果方法线性范围莠去津和苯并（a）芘分别为50~800μg/L、5.19~83.04μg/L,r均〉0.999;方法检出限分别为0.01、0.000 5μg/L;定量限为0.03、0.001 5μg/L;莠去津和苯并（a）芘在水中平均回收率分别为88.9%和90.4%;RSD均〈6.8%。结论该方法准确灵敏、精密度好,适用于水样中莠去津和苯并（a）芘测定。

Objective Establish a solid phase extraction-high performance liquid chromatography（ HPLC） analysis method with double detector to simultaneously determine atrazine and benzo（ a） pyrene in water. Methods Atrazine and benzo（ a） pyrene. In was enriched by large volume solid-phase extraction column and eluted by methylene chloride.Gradient elution was performed on Eclipe XDB-C18（ 150 mm × 4. 6 mm,5 μm） analytical column with acetonitrile-water as mobile phase. The products were then determined by diode array detector（ DAD） and fluorescence detector（ FLD）.Results The linear range of the method of atrazine and benzo（ a） pyrene was 50-800 μg / L and 5. 19-83. 04 μg / L,respectively. The correlation coefficients（ r） were both over 0. 999. The limits of detection were 0. 01 and 0. 000 5 μg / L,the limits of quantification were 0. 03 and 0. 001 5 μg / L,and the average recoveries of atrazine and benzo（ a） pyrene in water were 88. 9% and 90. 4%,respectively. The relative standard deviation was less than 6. 8%. Conclusion This method was accurate,sensitive and applicable for determining atrazine and benzo（ a） pyrenein water samples.