期刊文献+

酶联免疫吸附法和DNA检测法在肉类鉴别中的应用 被引量:28

Application of ELISA and DNA detection methods in meat identification
原文传递
导出
摘要 肉类食品是我国人民食品消费的主要组成部分,企业和经营单位为了追逐利润,时有将价格便宜的肉品掺入或替代高价格的肉品中进行加工和销售。为保护消费者对食品消费的知情权和规范肉类市场的秩序,食品生产、流通等监管部门应对肉类识别技术进行系统的分析和研究,进而建立相应的规范识别方法。目前用于肉类掺假鉴别的方法有组织学、化学、免疫学和基于DNA序列的检测方法等。本文主要针对ELISA检测方法和DNA检测方法在肉类识别中的应用进行阐述分析,并对我国肉类识别标准方法的缺陷进行分析。 Meat is the main component of food consumption. In order to make profit,companies may adulterate cheap meat to expensive meat during processing and marketing. To protect the consumer’s right and regulate meat market,supervision agencies should systematically analyze meat identification technology and establish an appropriate methods.Currently,the methods of meat identification include histological methods,chemical methods,immunological methods and DNA sequence-based detection methods. This paper focuses on the application of ELISA and DNA detection methods in meat identification. Besides,the defects of GB standards were also analyzed.
出处 《中国食品卫生杂志》 2015年第1期93-97,共5页 Chinese Journal of Food Hygiene
关键词 酶联免疫吸附法 DNA 肉类 掺假 鉴别 食品安全 ELISA DNA meat adulteration identification food safety
  • 相关文献

参考文献33

  • 1All M E,Hashim U,Dhahi T S,et al. Analysis of pork adulteration in commercial burgers targeting porcine-speciflc mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction [ J ]. Food Analytie,,d Methods ,2012,5 (4) :784-794.
  • 2孙秀兰,管露,单晓红,张银志.食品过敏原体外检测方法研究进展[J].东北农业大学学报,2012,43(2):126-132. 被引量:15
  • 3徐霞,成芳,应义斌.近红外光谱技术在肉品检测中的应用和研究进展[J].光谱学与光谱分析,2009,29(7):1876-1880. 被引量:78
  • 4Rogberg-Mufioz A,Posik D M, Rlpoli M V, et al. Recent patents for detecting the species of origin in anintl feedstuff, and raw and processed neat prodnctsJ].Recent Pat Food Nutr Agric,2013,5( 1 ) :3-8.
  • 5Arif 1 A, Khan H A. Molecular markers for biodiversity analysis of wildlife animals: a brief review [ J ]. Animal Biodiversity andConservation ,2009,32 ( 1 ) :9-17.
  • 6USDA,Food Safety and Inspection Service, Office of Public Health and Science,et al. Laboratory guidebook notice of change[ EB/OL]. [2013-104)6]. http ://www. doc88, com/p-676129338626, html.
  • 7冯永巍,王琴.肉类掺假检验技术研究进展[J].食品与机械,2013,29(4):237-240. 被引量:24
  • 8Sherikar A T, Karkare U D, Khot J B, et am. Studies on thermostable antigens, production of species-specific antiadrenal sera and comparison of immunological techniques in meat speciation[ J]. Meat Sci,1993,33 (1) :121-136.
  • 9Myers M J ,Yancy H F,Farrell D E,et al. Assessment of two enzyme- linked immunosorbent assay tests marketed for deteetion of ruminant proteins in finished feed [ J ]. J Food Prot ,2007,70 ( 3 ) :692-699.
  • 10RAO Q, Hsieh Y H P. Competitive enzyme-linked immunosorbent assay for quantitative detection of bovine blood in heat-processed meat and feed[J]. J Food Prot,2008,71 (5) :1000-1006.

二级参考文献141

共引文献196

同被引文献316

引证文献28

二级引证文献175

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部