汉麻叶总黄酮和总多酚的同步提取工艺优化及抗氧化活性

Optimized methodology for the simultaneous extraction of total flavonoids, total phenolic compounds and antioxidant capacity from Hemp leaves

在单因素试验基础上,以汉麻叶总黄酮、总多酚提取率和对DPPH自由基的清除率为响应值,利用响应面分析法对汉麻叶总黄酮、总多酚的提取工艺进行同步优化。研究表明,乙醇体积分数、提取时间、提取温度和液料比4个因素对总黄酮、总多酚的提取率均有显著影响。根据响应面法绘制三维及等高线叠加图,应用同步优化程序,得出总黄酮、总多酚的最佳提取工艺参数为乙醇体积分数65%、提取时间133 min、提取温度60℃、液料比30:1(m L/g)。在此条件下进行验证试验,得到汉麻叶总黄酮、总多酚提取率和对DPPH自由基的清除率分别为(2.01±0.22)%、(1.84±0.32)%、(77.63±2.15)%,与数学模型计算所得预测值接近。可见,响应面同步优化法对汉麻叶总黄酮、总多酚提取条件进行同时优化合理可行。

Response surface methodology was used to optimize the simultaneous extraction of total flavonoids（TFs） and total phenolics compounds（TPC） from Hemp leaves based on extraction rate and their scavenging capacity against DPPH free radicals. All four operating parameters including ethanol concentration, extraction time, extraction temperature and solvent-to-solid ratio had a significant effect on the extraction rate of TFs and TPC. The optimum process conditions for simultaneously increasing the yields of TFs and TPC were found to be extraction at 60 ℃ for 133 min with an ethanol concentration of 65% and a solvent-to-solid ratio of 30：1（m L/g）. The actual extraction rate and their scavenging capacity of TFs and TPC under the optimized conditions were（2.01±0.22）%,（1.84±0.32）%,（77.63±2.15）%, respectively. Good agreement with the predicted values was found. Therefore, response surface methodology is a feasible way to optimize the simultaneous extraction of TFs and TPC from Hemp leaves.