Livin、Caspase-3蛋白在不同胃病变组织中的表达及其临床意义

Expressions and Clinical Significances of Livin and Caspase-3 Protein in Different Kinds of Gastric Lesions

目的探讨Livin、Caspase-3蛋白在不同胃病变组织中的表达及其临床意义。方法选择2013年3月～2014年3月在哈里逊国际和平医院确诊为慢性非萎缩性胃炎（chronic non atrophic gastritis）的患者20例、慢性萎缩性胃炎（chronic atrophic gastritis,CAG）非肠化的患者30例、CAG伴肠化的患者30例以及胃癌患者30例（其中低分化组10例、中分化组10例、高分化组10例）为研究对象,应用免疫组织化学法（IHC）检测和比较各组患者胃病变组织中Livin、Caspase-3蛋白的表达。结果慢性非萎缩性胃炎、CAG非肠化、CAG伴肠化和胃癌组织中Livin蛋白的阳性表达率分别为0%、40%、43.33%、60%,差异均有统计学意义（P〈0.001）,低、中、高分化胃癌组织中Livin蛋白的阳性表达率比较,差异均无统计学意义（P〉0.05）;慢性非萎缩性胃炎、CAG非肠化、CAG伴肠化、胃癌组织中Caspase-3蛋白的阳性表达率分别为90%、53.33%、46.67%、30%,差异均有统计学意义（P〈0.001）,低、中、高分化胃癌组织中Caspase-3蛋白的阳性表达率比较,差异均无统计学意义（P〉0.05）。胃癌组织中,Livin、Caspase-3蛋白的表达呈显著负相关（r=-0.505,P〈0.05）。结论 Caspase-3与Livin蛋白的表达失调促进了胃癌的发生,二者可能有助于早期诊断和预防胃癌。

Objective To investigate the expressions and clinical significances of livin and caspase-3 protein in different kinds of gastric lesions. Methods We selected 110 cases of gastric patients who were admitted in Harrison Hospital of Hengshui City between March 2013 and March 2014 as the research objects. There were 20 cases of patients with chronic non atrophic gastritis, 30 cases of patients with chronic atrophic gastritis（CAG） but without intestinal metaplasia, 30 cases of patients with CAG and intestinal metaplasia, and 30 cases of patients with gastric carcinoma, including 10 cases of lowly differentiated gastric carcinoma, 10 cases of middle differentiated gastric carcinoma and 10 cases of highly differentiated gastric carcinoma. The livin, caspase-3 protein expressions were detected by immunohistochemical assay（IHC）. Results The expression rate of livin protein in chronic non atrophic gastritis, CAG without intestinal metaplasia, CAG with intestinal metaplasia, gastric carcinoma were respectively 0%, 40%, 43.33%, 60%, and the difference was statistically significant（P〈0.001）. No statistical difference was found in the expression of livin protein between lowly, middle and highly differentiated gastric carcinoma（P〈0.05）. The expression rate of caspase-3 protein in chronic non atrophic gastritis, CAG without intestinal metaplasia, CAG with intestinal metaplasia, gastric carcinoma were respectively 90%, 53.33%, 46.67%, 30%, and the difference was statistically significant（P〈0.001）; no statistical difference was found in the expression of caspase-3 protein between lowly, middle and highly differentiated gastric carcinoma（P〈0.05）. The expression of caspase-3 protein was negatively related to the expression of livin in gastric carcinoma（r=-0.505, P〈0.05）. Conclusion The abnormal expressions of livin and caspase-3 contributed to the development of gastric carcinoma. Both could be helpful in the early diagnosis and pretreatment of gastric carcinoma.