苯甲酸钠多次给药对大鼠肝肾功能的影响

Effects of sodium benzoate on liver and kidney in rats

目的:研究食品防腐剂苯甲酸钠多次给药对大鼠肝肾功能的影响。方法:选取50只健康SPF级SD大鼠,分为5组,每组10只,苯甲酸钠低、中、高剂量组分别灌胃给予苯甲酸钠(0.1、0.2、0.4 g/kg),阳性对照组给予山梨酸钾(0.2 g/kg),空白对照组给予生理盐水,1次/d,连续4周。检测末次给药后大鼠血清学指标(ALT、AST、ALP、GGT、TP、ALB、TBIL、BUN、Cre、UA),并观察肝肾组织病理学改变。结果:与空白对照组相比,苯甲酸钠低、中、高剂量组大鼠的各项血清生化学指标均有一定的异常,高剂量组最为显著(P均<0.05)。苯甲酸钠高剂量组大鼠肝脏出现了明显病理学异常,各组大鼠肾脏均未见病理学改变。结论:苯甲酸钠多次给药对大鼠肝肾,尤其是肝脏有一定的损害。

Aim： To study the effects of sodium benzoate on liver and kidney in rats.Methods： Fifty healthy SD rats were allocated into 5 groups and treated with sodium benzoate（ 0.1,0.2,0.4 g / kg）,potassium sorbate（ 0.2 g / kg） and normal saline by gavage for 4 weeks.The serum indicators such as ALT,AST,ALP,GGT,TP,ALB,TBIL,BUN,Cre,and UA were determined and the liver and renal histology of the rats was observed.Results： Rats in each dose group of sodium benzoate had abnormal serum biochemical indicators,especially in high dose group.Furthermore,rats in sodium benzoate high dose group showed obvious pathological abnormalities in liver.Rats in all groups showed no pathological changes in kidneys.Conclusion： The frequent use of sodium benzoate could do harms to the kidney and liver especially to the liver in rats.