超微铁皮石斛对脾虚便秘小鼠肠道细菌分子多样性的影响

Effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation

探讨超微铁皮石斛对脾虚便秘肠道细菌分子多样性的影响,可为其疗效和临床应用提供依据.制备小鼠脾虚便秘模型,设模型组、正常组、铁皮石斛传统汤剂治疗组和超微50%量汤剂治疗组,采集肠道内容物,提取肠道微生物宏基因组,通过细菌通用引物PCR扩增后用ARDRA分析其多样性.结果表明:正常组和铁皮石斛超微50%量汤剂组的OTUs(分类操作单元数)、Shannon指数和Brillouin指数分别相同,依次为6、2.5850和1.5820,模型组和铁皮石斛传统汤剂组的OTUs、Shannon指数和Brillouin指数也分别相同,依次为5、2.3219和1.3814,且前两组均大于后两组;铁皮石斛超微50%量汤剂组与正常组群落结构的相似性系数最大,为0.5000,模型组和传统汤剂组较小,为0.1818;聚类分析和主成分分析结果均显示,铁皮石斛超微50%量汤剂组小鼠肠道细菌分子多样性最接近正常组.因此,脾虚便秘造模使小鼠肠道细菌多样性遭到破坏,经铁皮石斛两种汤剂治疗,具有一定的调整作用,超微50%量汤剂效果优于传统汤剂.

This research aimed to study the effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation,in order to provide scientific basis for ultra-micro D.officinale therapy.The mice with spleen-deficiency constipation in treatment groups were respectively given the traditional decoction of D.officinale and 50% dose ultra-micro powder D.officinale.The metagenome DNA of intestinal flora was extracted from intestinal contents.Then ARDRA analysis was made after PCR by universal primer of bacteria.The results showed that OTUs,Shannon index and Brillouin index were the same between the normal group and the 50% dose ultra-micro powder group,and between the model group and the traditional decoction group,but greater in the former two groups than the latter ones.The similarity coefficient（0.5000） was bigger between the 50% dose ultra-micro powder group and the normal group than between the model group and the traditional decoction group,which was 0.181 8.Both cluster analysis and principal components analysis showed that the molecular diversity of intestinal microbiotia in mice of 50% dose ultra-micro powder group was the closest to that in the normal group.The results showed that the weakened molecular diversity of intestinal bacteria in mice with spleen-deficiency constipation can be regulated by the two kinds of D.officinale treatments to different extent.The efficacy of 50% dose ultra-micro powder D.officinale is better than the traditional decoction.